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. 2023 Sep 12;7(17):4701-4704.
doi: 10.1182/bloodadvances.2022008033.

Flow cytometry as a fast, cost-effective tool to assess IGHV mutational status in CLL

Guillaume Couillez  1 , Pierre Morel  2 , Valentin Clichet  1 , Ludivine Fourdrain  1 , Caroline Delette  2 , Véronique Harrivel  1 , Brigitte Gubler  3   4 , Camille Rottier  3 , Sophie Derreumaux  5 , Emilie Margat  6 , Loic Garcon  1   4 , Jean-Pierre Marolleau  2   4 , Thomas Boyer  1   4
Affiliations

Flow cytometry as a fast, cost-effective tool to assess IGHV mutational status in CLL

Guillaume Couillez et al. Blood Adv. .
No abstract available

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Conflict of interest statement

Conflict-of-interest disclosure: The authors declare no competing financial interests.

Figures

Figure 1.
Figure 1.
Gating strategy and naive/memory B-cell profile in MFC. (A) Lymphocytes were gated as CD45+/SS low cells. (B) Then, CLL cells were gated as CD19+/CD5+ lymphocytes. Normal residual B cells are CD19+/CD5− lymphocytes. (C) Naive (IgD+/CD27−) and memory (IgD−/CD27+) status of B-cell lymphocytes was finally determined.
Figure 2.
Figure 2.
Distribution of B-cell profiles according to IGHV mutational status. Plot showing the distribution between naive B-cell or memory B-cell profiles according to IGHV mutational status (0 = unmutated; 1 = mutated).
See this image and copyright information in PMC

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