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. 2022 Oct 14;11(10):1415.
doi: 10.3390/antibiotics11101415.

Ribes nigrum L. Extract-Mediated Green Synthesis and Antibacterial Action Mechanisms of Silver Nanoparticles

Zaruhi Hovhannisyan  1 , Marina Timotina  2 , Jemma Manoyan  3 , Lilit Gabrielyan  3 , Margarit Petrosyan  3 , Barbara Kusznierewicz  4 , Agnieszka Bartoszek  4 , Claus Jacob  1 , Mikayel Ginovyan  3   5 , Karen Trchounian  3   5 , Naira Sahakyan  3   5 , Muhammad Jawad Nasim  1
Affiliations

Ribes nigrum L. Extract-Mediated Green Synthesis and Antibacterial Action Mechanisms of Silver Nanoparticles

Zaruhi Hovhannisyan et al. Antibiotics (Basel). .

Abstract

Silver nanoparticles (Ag NPs) represent one of the most widely employed metal-based engineered nanomaterials with a broad range of applications in different areas of science. Plant extracts (PEs) serve as green reducing and coating agents and can be exploited for the generation of Ag NPs. In this study, the phytochemical composition of ethanolic extract of black currant (Ribes nigrum) leaves was determined. The main components of extract include quercetin rutinoside, quercetin hexoside, quercetin glucuronide, quercetin malonylglucoside and quercitrin. The extract was subsequently employed for the green synthesis of Ag NPs. Consequently, R. nigrum leaf extract and Ag NPs were evaluated for potential antibacterial activities against Gram-negative bacteria (Escherichia coli ATCC 25922 and kanamycin-resistant E. coli pARG-25 strains). Intriguingly, the plant extract did not show any antibacterial effect, whilst Ag NPs demonstrated significant activity against tested bacteria. Biogenic Ag NPs affect the ATPase activity and energy-dependent H+-fluxes in both strains of E. coli, even in the presence of N,N'-dicyclohexylcarbodiimide (DCCD). Thus, the antibacterial activity of the investigated Ag NPs can be explained by their impact on the membrane-associated properties of bacteria.

Keywords: Ribes nigrum; antimicrobial; natural products; phytochemical investigation; silver nanoparticles.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
HPLC profiles of extracts from R. nigrum recorded before (270 nm, Panel A) and after (734 nm, Panel B) post-column derivatization with ABTS. For identity of peaks, see Table 1.
Figure 2
Figure 2
UV-Vis spectrum of reaction mixture containing biosynthesized Ag NPs.
Figure 3
Figure 3
The change in the color of nano-suspensions with the passage of time.
Figure 4
Figure 4
SEM images of Ag NPs (panels a and b). TEM Images of Ag NPs (panels c and d). See text for details.
Figure 5
Figure 5
Impact of biogenic Ag NPs on specific growth rate of E. coli ATCC 25922 and kanamycin-resistant E. coli pARG-25 strains.
Figure 6
Figure 6
The impact of biogenic Ag NPs on ATPase activity of E. coli ATCC 25922 and kanamycin-resistant E. coli pARG-25 membrane vesicles.
Figure 7
Figure 7
The effect of biogenic Ag NPs on H+-fluxes through the E. coli ATCC 25922 and E. coli pARG-25 membranes.
Figure 8
Figure 8
Schematic presentation of possible action mechanisms of Ag NPs on E. coli.
See this image and copyright information in PMC

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