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Review
. 2022 Oct 14;11(20):3235.
doi: 10.3390/cells11203235.

The Power of Gene Technologies: 1001 Ways to Create a Cell Model

Maxim Karagyaur  1   2 , Alexandra Primak  2 , Anastasia Efimenko  1   2 , Mariya Skryabina  2 , Vsevolod Tkachuk  1   2
Affiliations
Review

The Power of Gene Technologies: 1001 Ways to Create a Cell Model

Maxim Karagyaur et al. Cells. .

Abstract

Modern society faces many biomedical challenges that require urgent solutions. Two of the most important include the elucidation of mechanisms of socially significant diseases and the development of prospective drug treatments for these diseases. Experimental cell models are a convenient tool for addressing many of these problems. The power of cell models is further enhanced when combined with gene technologies, which allows the examination of even more subtle changes within the structure of the genome and permits testing of proteins in a native environment. The list and possibilities of these recently emerging technologies are truly colossal, which requires a rethink of a number of approaches for obtaining experimental cell models. In this review, we analyze the possibilities and limitations of promising gene technologies for obtaining cell models, and also give recommendations on the development and creation of relevant models. In our opinion, this review will be useful for novice cell biologists, as it provides some reference points in the rapidly growing universe of gene and cell technologies.

Keywords: CRISPR/Cas9; cell models; cis-/transgene expression; gene technologies; genome editing; primary cell cultures; trans-splicing.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Cell-based models: advantages, limitations, and potential applications. Created with BioRender.com.
Figure 2
Figure 2
Main genetic engineering technologies for cell-based modelling: principle, advantages, limitations, and potential applications: (A)—cis-/transgene expression, (B)—genome editing, (C)—trans-splicing. Created with BioRender.com.
See this image and copyright information in PMC

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