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. 2022 Oct 6;12(10):1553.
doi: 10.3390/life12101553.

The Antioxidant, Analgesic, Anti-Inflammatory, and Wound Healing Activities of Haplophyllum tuberculatum (Forsskal) A. Juss Aqueous and Ethanolic Extract

Abdelkrim Agour  1 , Ibrahim Mssillou  1 , Imane Es-Safi  2 , Raffaele Conte  3 , Hamza Mechchate  2 , Meryem Slighoua  2 , Fatima Ez-Zahra Amrati  2 , Mohammad Khalid Parvez  4 , Omer Numan  4 , Amina Bari  2 , Badiaa Lyoussi  1 , Elhoussine Derwich  1
Affiliations

The Antioxidant, Analgesic, Anti-Inflammatory, and Wound Healing Activities of Haplophyllum tuberculatum (Forsskal) A. Juss Aqueous and Ethanolic Extract

Abdelkrim Agour et al. Life (Basel). .

Abstract

Herbal extracts are part of the solution to the increased demand for organic health care products. Traditionally, the different extracts prepared from Haplophyllum tuberculatum (Forsskal) A. Juss (H. tuberculatum) have been widely used to treat a wide range of illnesses. The aim of this study is to evaluate the antioxidant, analgesic, anti-inflammatory, and wound healing potential of the aqueous (HTAE) and ethanolic (HTEE) extracts of this plant as well as identify its major phytochemical components using LC-MS. Phytochemical analysis of both extracts revealed a rich composition and especially high amounts of glycosylic flavonols, 65.37% and 68.77% for the HTEE and HTAE, respectively. The antioxidant assays performed (DPPH, FRAP and TAC) indicated the excellent activity of the ethanolic extract while the in vivo activities (analgesic, anti-inflammatory, and healing potential) indicated the excellent activity of the aqueous extract. These findings support the therapeutic use of this plant by preventing pain and inflammation and promoting wound healing. To uncover, identify, and isolate compounds of potential medicinal and therapeutic significance, more studies on this species are required.

Keywords: H. tuberculatum; analgesic; anti-inflammatory; antioxidant; wound healing.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
(A) H. tuberculatum flowers; (B) stems and leaves of H. tuberculatum.
Figure 2
Figure 2
HTEE (1) and HTAE (2) extract ointment from H. tuberculatum.
Figure 3
Figure 3
Analgesic activity of H. tuberculatum extracts and the standard compound tramadol. Data are expressed as means ± SEM (n = 5). *** p < 0.001 compared to both plant extracts.
Figure 4
Figure 4
Anti-inflammatory activity of H. tuberculatum extracts and the standard compound. (T3: 3rd hour; T4: 4th hour; T5: 5th hour and T6; 6th hour); Data are expressed as mean as means ± SEM (n = 5). * p < 0.05 and ** p < 0.01 compared to Indomethacin.
Figure 5
Figure 5
Percentage of wound contraction by using H. tuberculatum extracts and indomethacin (standard). Data are expressed as means ± SEM (n = 4). * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to negative control.
Figure 6
Figure 6
Images of the healing process in treated rats.
See this image and copyright information in PMC

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