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Randomized Controlled Trial
. 2022 Oct 14;14(20):4300.
doi: 10.3390/nu14204300.

Effects of Lactiplantibacillus plantarum OLL2712 on Memory Function in Older Adults with Declining Memory: A Randomized Placebo-Controlled Trial

Keisuke Sakurai  1 , Takayuki Toshimitsu  2 , Erika Okada  1 , Saya Anzai  1 , Izumi Shiraishi  1 , Noriko Inamura  3   4 , Satoru Kobayashi  4 , Toshihiro Sashihara  2 , Tatsuhiro Hisatsune  1
Affiliations
Randomized Controlled Trial

Effects of Lactiplantibacillus plantarum OLL2712 on Memory Function in Older Adults with Declining Memory: A Randomized Placebo-Controlled Trial

Keisuke Sakurai et al. Nutrients. .

Abstract

The use of probiotics is expected to be an intervention in neurodegenerative conditions that cause dementia owing to their ability to modulate neuroinflammatory responses via the microbiome-gut-brain axis. Therefore, we selected Lactiplantibacillus plantarum OLL2712 (OLL2712), the optimal anti-inflammatory lactic acid bacteria strain with high IL-10-inducing activity in immune cells, and aimed to verify its protective effects on memory function in older adults. A 12-week, randomized, double-blind, placebo-controlled trial was performed with older adults over the age of 65 years with declining memory. The participants consumed either powder containing heat-treated OLL2712 cells or placebo. Memory function was assessed using a computer-assisted cognitive test, Cognitrax. Daily dietary nutrient intake was assessed using the Brief-type Self-administered Diet History Questionnaire (BDHQ). The composition of the gut microbiota was analyzed by fecal DNA extraction and 16S rDNA sequencing. Data from 78 participants who completed the entire procedure were analyzed, and significant improvements in composite memory and visual memory scores were observed in the active group, after accounting for the effect of daily nutritional intake (p = 0.044 and p = 0.021, respectively). In addition, the active group had a lower abundance ratio of Lachnoclostridium, Monoglobus, and Oscillibacter genera, which have been reported to be involved in inflammation. The present study suggests that OLL2712 ingestion has protective effects against memory function decline in older adults.

Keywords: Lactiplantibacillus; aging; gut microbiota; memory; mild cognitive impairment; probiotics.

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Conflict of interest statement

T.T. and T.S. are employees of Meiji Co., Ltd. The other authors, K.S., E.O., S.A., I.S., N.I., S.K. and T.H. report no conflicts of interest in this work.

Figures

Figure 1
Figure 1
Flow chart showing the number of participants in the active and placebo groups at each stage of the study.
Figure 2
Figure 2
The composition of the gut microbiota at the genus level. Taxa with low relative abundances of <1% are included in others.
Figure 3
Figure 3
The relative abundance of Lachnoclostridium, Monoglobus, and Oscillibacter shown in Box-and-whisker diagram. * p < 0.05.
See this image and copyright information in PMC

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