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. 2022 Oct 17;8(10):e11125.
doi: 10.1016/j.heliyon.2022.e11125. eCollection 2022 Oct.

Isolation, characterization and anti-UVB irradiation activity of an extracellular polysaccharide produced by Lacticaseibacillus rhamnosus VHPriobi O17

Shudong Peng  1   2 , Chaoqun Guo  3 , Songjie Wu  3 , Zhi Duan  3
Affiliations

Isolation, characterization and anti-UVB irradiation activity of an extracellular polysaccharide produced by Lacticaseibacillus rhamnosus VHPriobi O17

Shudong Peng et al. Heliyon. .

Abstract

The purpose of this study was to isolate exopolysaccharides (EPS) from lactic acid bacteria (LAB) and evaluate EPS anti-UVB viability. Lacticaseibacillus rhamnosus VHPriobi O17 with high EPS production was screened from 34 strains of LAB. The EPS (OP-2) produced by L. rhamnosus VHPriobi O17 was purified by alcohol precipitation and DEAE-μSphere anion exchange chromatography. By ion chromatography, FT-IR spectrum and gel column chromatography, EPS (OP-2) was a novel Man-like polysaccharide with the weight-averaged molecular of 84.2 kDa. The EPS (OP-2) can effectively alleviate HaCaT cells apoptosis and overproduction of reactive oxygen species (ROS) induced by UVB. The results also showed that it inhibited the release of pro-inflammatory cytokines (IL-1α, IL-6 and IL-8); and suppressed the phosphorylation cascade of JNK and p38 MAPK to reduce the expression level of active-caspase3, ultimately prevented cell apoptosis. Thus, the EPS produced by L. rhamnosus VHPriobi O17 have the potential to be used for human anti-UVB irradiation.

Keywords: Anti-UVB; Exopolysaccharides; HaCaT cells; Lacticaseibacillus rhamnosus.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Identification of the Lacticaseibacillus rhamnosus VHPriobi O17. A, Colonial morphology of L. rhamnosus VHPriobi O17. B, Gram staining of L. rhamnosus VHPriobi O17. C, Mass spectrum of L. rhamnosus VHPriobi O17 by MALDI-TOF MS. D, Phylogenetic tree of L. rhamnosus VHPriobi O17 according to the 16S rDNA sequence.
Figure 2
Figure 2
A, growth curve and exopolysaccharides (EPS) production of L. rhamnosus VHPriobi O17. B, chromatographic diagram of EPS separated by DEAE-μSphere anion exchange Column. Data are presented as the mean ± standard deviation (n = 3).
Figure 3
Figure 3
Structure characterization of EPS (OP-2) produced by L. rhamnosus VHPriobi O17. A, monosaccharide composition. B, FT-IR spectra. C, Gel column chromatogram. D, a standard curve of average molecular weight of the GPC method.
Figure 4
Figure 4
A, Toxicity of EPS (OP-2) purified from L. rhamnosus VHPriobi O17 to HaCaT cells. B-D, protective effects of EPS (OP-2) on HaCaT cells exposed to UVB (radiation doses are 7.5 mJ/cm2, 15 mJ/cm2, and 30 mJ/cm2, respectively). Data are presented as the mean ± standard deviation (n = 3). Mean values with different letters are significantly different (p < 0.05).
Figure 5
Figure 5
Effects of purified EPS (OP-2) from L. rhamnosus VHPriobi O17 on intracellular ROS (A), IL-1α (B), IL-1β (C), IL-6 (D), and IL-8 (E) in HaCaT cells exposed to UVB at 15 mJ/cm2. Data are presented as the mean ± standard deviation (n = 3). Mean values with different letters are significantly different (p < 0.05).
Figure 6
Figure 6
Effects of purified EPS (OP-2) from L. rhamnosus VHPriobi O17 on the expression in HaCaT cells exposed to UVB at 15 mJ/cm2. A, Representative Western blot for phospho-JNK, phospho-p38 MAPK, and active-caspase3 in cells. B, Quantification of phospho-JNK, phospho-p38 MAPK, and active-caspase3 blots. Data are presented as the mean ± standard deviation (n = 3). Significant differences (p < 0.05) are indicated by different letters. The uncropped images of (A) were referred to in supplementary F 1.
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References

    1. Verschooten L., Claerhout S., Van Laethemii A., Agostinis P., Garmyn M. Invited review new strategies of photoprotection. Photochem. Photobiol. 2006;82:1016–1023. - PubMed
    1. Salucci S., Burattini S., Battistelli M., Baldassarri V., Maltarello M.C., Falcieri E. Ultraviolet B (UVB) irradiation-induced apoptosis in various cell lineages in vitro. Int. J. Mol. Sci. 2013;14:532–546. - PMC - PubMed
    1. Kammeyer A., Luiten R.M. Oxidation events and skin aging. Ageing Res. Rev. 2015;21:16–29. - PubMed
    1. Liu W., Wang F., Li C., Otkur W., Hayashi T., Mizuno K., Hattori S., Fujisaki H., Onodera S., Ikejima T. Silibinin treatment protects human skin cells from UVB injury through upregulation of estrogen receptors. J. Photochem. Photobiol. B Biol. 2021;216 - PubMed
    1. Xiao Z., Yang S., Chen J., Li C., Zhou C., Hong P., Sun S., Qian Z.J. Trehalose against UVB-induced skin photoaging by suppressing MMP expression and enhancing procollagen I synthesis in HaCaT cells. J. Funct.Foods. 2020;74