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. 2022 Oct 10:10:1019663.
doi: 10.3389/fchem.2022.1019663. eCollection 2022.

Protective effects of silibinin on LPS-induced inflammation in human periodontal ligament cells

Di Meng  1 , Yuling Wang  1   2 , Tongjun Liu  1
Affiliations

Protective effects of silibinin on LPS-induced inflammation in human periodontal ligament cells

Di Meng et al. Front Chem. .

Abstract

Clinically, periodontitis is a chronic nonspecific inflammation that leads to damaged teeth and their supporting gum tissues. Although many studies on periodontitis have been conducted, therapy with natural products is still rare. Silibinin has been proven to have anti-inflammatory and antioxidant activities. However, the effects of silibinin on lipopolyssacharide (LPS)-induced inflammation in periodontal ligaments (PDLs) have not yet been investigated. In this study, the PDLs were treated with silibinin (10, 20, and 40 μM) in the presence of LPS. The results showed that silibinin treatment reduced the levels of NO, PGE2, IL-6, TNF-α, MMP-1, and MMP-3 and enhanced the activities of superoxide dismutase (SOD) and glutathione (GSH). Moreover, silibinin treatment downregulated RANKL levels and upregulated OPG and ALP levels. In summary, silibinin protected PDLs against LPS-induced inflammation, oxidative stress, and osteogenic differentiation.

Keywords: LPS-induced; human periodontal ligament cells; inflammation; protective effects; silibinin.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Chemical structure of SB.
FIGURE 2
FIGURE 2
Cytotoxicity assay of SB.
FIGURE 3
FIGURE 3
SB treatment reduced LPS-induced NO (A) and PGE2 (B) in hPDLs. # p < 0.05, compared to the LPS group.
FIGURE 4
FIGURE 4
SB treatment inhibited LPS-induced IL-6 (A) and TNF-α (B) in hPDLs. # p < 0.05, compared to the LPS group.
FIGURE 5
FIGURE 5
SB treatment inhibited LPS-induced MMP-1 (A) and MMP-3 (B) in hPDLs. # p < 0.05, compared to the LPS group.
FIGURE 6
FIGURE 6
SB treatment regulated LPS-induced SOD (A) and GSH (B) in hPDLs. # p < 0.05, compared to the LPS group.
FIGURE 7
FIGURE 7
SB treatment regulated LPS-induced SOD (A) and GSH (B) in hPDLs. # p < 0.05, compared to the LPS group.
FIGURE 8
FIGURE 8
SB treatment regulated LPS-induced ALP in hPDLs. # p < 0.05, compared to the LPS group.
FIGURE 9
FIGURE 9
Effect of SB on LPS-induced hPDLs.
See this image and copyright information in PMC

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