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Case Reports
. 2022 Dec;12(12):330.
doi: 10.1007/s13205-022-03395-w. Epub 2022 Oct 24.

Insights from the genome sequence of Bacillus tropicus EMB20, an efficient β-lactamase-producing bacterium

Huma Fatima  1 , Sumit Kumar  1   2 , Sunil Kumar Khare  1
Affiliations
Case Reports

Insights from the genome sequence of Bacillus tropicus EMB20, an efficient β-lactamase-producing bacterium

Huma Fatima et al. 3 Biotech. 2022 Dec.

Abstract

We report here the whole-genome sequence of β-lactamase-producing bacteria Bacillus tropicus EMB20. The genome sequence of Bacillus tropicus EMB20 has a size of 5.8 Mb (G + C content of 35.52%) with 5593 coding DNA sequences (CDSs), 108 tRNA, and 14 rRNA operons. The bacterium has the unique ability to produce a β-lactamase enzyme with high activity. β-Lactamases are one of the most common causes of antimicrobial resistance as these enzymes inactivate almost all β-lactam antibiotics. The antibiotic susceptibility test showed that the B. tropicus EMB20 is producing β-lactamase and can degrade the β-lactam antibiotics. Further, the antibiotic degradation potential of this bacteria was confirmed by growing the bacteria in the presence of varying concentrations of β-lactam antibiotic, amoxicillin. The bacteria were able to hydrolyze amoxicillin up to 50 mg/L in 4 h. Furthermore, the analyses of the genome revealed the presence of multiple β-lactamase genes, possibly involved in antibiotic degradation. The availability of the genome sequence will provide further insights into the mechanism of antimicrobial resistance by β-lactamase-producing bacteria.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-022-03395-w.

Keywords: Antimicrobial resistance; Bacillus tropicus; Genome sequence; β-Lactamase.

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Conflict of interest statement

Conflict of interestThe authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1
Fig. 1
Genome sequencing of B. tropicus EMB20. A Comparative genomic analysis. B Average nucleotide identity phylogenetic tree. C RAST subsystem statistics of Bacillus tropicus EMB20
Fig. 1
Fig. 1
Genome sequencing of B. tropicus EMB20. A Comparative genomic analysis. B Average nucleotide identity phylogenetic tree. C RAST subsystem statistics of Bacillus tropicus EMB20
Fig. 2
Fig. 2
Antibiotic susceptibility test using Octa disc Combi 67 having antibiotics ampicillin (A), ampicillin/sulbactam (As), amoxicillin/clavulanic acid (Ac), Piperacillin/Tazobactam (Pt), Ticarcillin/clavulanic acid (Tc), Cefoperazone/sulbactam (Cfs), cefuroxime (Cu) and Cefotaxime (Ce)
Fig. 3
Fig. 3
High-performance liquid chromatography (HPLC) analysis for amoxicillin degradation by B. tropicus EMB20. A spectrum of the standard amoxicillin (50 mg/L); B cell-free supernatant of B. tropicus EMB20 in media containing 50 mg/L amoxicillin at 0 h; C 2 h and D 4 h
See this image and copyright information in PMC

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