Kinetics of humoral response during the acute and the convalescent phase of human trichinosis

Abstract

After a common source outbreak of trichinosis in Bitburg, FRG, 107 patients, for whom time and source of infection were precisely known, were prospectively studied for a period of eight months in order to investigate the kinetics of the humoral immune response. Antibodies were assessed by IHA, and by ELISA to quantify IgG, IgM and IgE isotypes. For four different periods of the observation time sensitivity and predictive value of tests to identify acute or convalescent trichinosis were determined. The results demonstrated characteristic kinetics of the different antibody isotypes and varying diagnostic value of the tests. Only IgG-ELISA and double-sandwich IgM-ELISA reliably identified diseased patients shortly after onset of clinical illness and showed a negative predictive value of almost 100% from seven weeks to eight months after infection. IgE antibodies and elevation of total serum IgE occurred only in about 20% of patients and showed no consistent pattern during the course of the disease. Circulating immune complexes were moderately increased four weeks after infection. They rapidly returned to normal values, after the symptoms of the acute phase had disappeared.