Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Apr;15(2):387-399.
doi: 10.1007/s12602-022-10000-2. Epub 2022 Oct 29.

Screening of Wild Lactic Acid Bacteria from Algerian Traditional Cheeses and Goat Butter to Develop a New Probiotic Starter Culture

Sawsen Hadef  1   2 , Tayeb Idoui  3 , Mohamed Sifour  4 , Magali Genay  5 , Annie Dary-Mourot  5
Affiliations

Screening of Wild Lactic Acid Bacteria from Algerian Traditional Cheeses and Goat Butter to Develop a New Probiotic Starter Culture

Sawsen Hadef et al. Probiotics Antimicrob Proteins. 2023 Apr.

Abstract

Twenty-five lactic acid bacterial (LAB) strains have been isolated from traditional goat butter and three types of cheese (dry Klila, frech Klila, and Bouhezza) and evaluated for technological abilities, probiotic properties, and potentials as starter cultures. The twenty-five LAB strains comprised eight strains belonging to Lactobacillus, four strains belonging to Lactococcus, eleven strains belonging to Enterococcus, and two strains belonging to Leuconostoc. A non-hierarchical cluster analysis was performed in order to select the performing strains. After carrying out the preliminary phenotypic characterizations and the probiotic potential, three strains designated as BM10, B15, and C30 belonging to the genus Lactobacillus and Enterococcus with good tolerance to acidity were selected. The strains showed a significant resistance to 0.5% bile salts and 0.4% phenol. Hemolytic activity was not detected; in addition, good hydrophobicity and autoaggregation was obtained. A significant antimicrobial activity was exhibited by all selected strains against Listeria innocua. Genotypic identification by 16S rRNA allowed the identification of B15, BM10, and C30 as Lactobacillus plantarum, Lactobacillus casei, and Enterococcus durans, respectively. The results of the current study suggest that the strains isolated from Algerian fermented dairy products have high potential as probiotic starter cultures in the goat butter and cheese industry.

Keywords: Goat butter; Lactic acid bacteria; Probiotic; Starter culture; Technological abilities; Traditional cheese.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Rizzoli R, Biver E (2017) Yogurt consumption and impact on bone health. In: Shah NP (ed) Yogurt in Health and Disease Prevention. Academic Press pp 507–524. https://doi.org/10.1016/B978-0-12-805134-4.00029-8
    1. Melini F, Melini V, Luziatelli F, Ficca AG, Ruzzi M (2019) Health-promoting components in fermented foods: an up-to-date systematic review. Nutrients 11:1189. https://doi.org/10.3390/nu11051189 - DOI - PubMed - PMC
    1. Parekh S, Vinci VA, Strobel RJ (2000) Improvement of microbial strains and fermentation processes. Appl Microbiol Biotechnol 54:287–301. https://doi.org/10.1007/s002530000403 - DOI - PubMed
    1. Rettedal EA, Altermann E, Roy NC, Dalziel JE (2019) The effects of unfermented and fermented cow and sheep milk on the gut microbiota. Front Microbiol 10. https://doi.org/10.3389/fmicb.2019.00458 - DOI - PubMed - PMC
    1. Kumar N, Raghavendra M, Tokas J, Singal HR (2017) Flavor addition in dairy products. In: Watson RR, Collier RJ, Preedy VR (ed) Nutrients in Dairy and Their Implications on Health and Disease. Academic Press pp 123–135. https://doi.org/10.1016/B978-0-12-809762-5.00010-3

LinkOut - more resources