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Meta-Analysis
. 2022 Oct 28;23(1):60.
doi: 10.1186/s12868-022-00734-8.

Sustained delivery of chABC improves functional recovery after a spine injury

Atousa Sharifi  1 , Ali Zandieh  1 , Zahra Behroozi  2   3 , Michael R Hamblin  4 , Sara Mayahi  5 , Mahmoud Yousefifard  6 , Fatemeh Ramezani  7
Affiliations
Meta-Analysis

Sustained delivery of chABC improves functional recovery after a spine injury

Atousa Sharifi et al. BMC Neurosci. .

Abstract

Introduction: Chondroitinase ABC (chABC) is an enzyme could improve regeneration and thereby improving functional recovery of spinal cord injury (SCI) in rodent models. Degradation of the active enzyme and diffusion away from the lesion are the causes of using hydrogels as a scaffold to deliver the chABC into the lesion site. In this meta-analysis, we investigated the effects of chABC embedded in a scaffold or hydrogel on the functional recovery after SCI.

Method: Databases were searched based on keywords related to chABC and spinal cord injury (SCI). Primary and secondary screening was performed to narrow down study objectives and inclusion criteria, and finally the data were included in the meta-analysis. The standard mean difference of the score of the functional recovery that measured by Basso, Beattie, Bresnahan (BBB) test after SCI was used to analyze the results of the reported studies. Subgroup analysis was performed based on SCI model, severity of SCI, transplantation type, and the follow-up time. Quality control of articles was also specified.

Results: The results showed that embedding chABC within the scaffold increased significantly the efficiency of functional recovery after SCI in animal models (SMD = 1.95; 95% CI 0.71-3.2; p = 0.002) in 9 studies. SCI model, severity of SCI, injury location, transplantation type, and the follow-up time did not affect the overall results and in all cases scaffold effect could not be ignored. However, due to the small number of studies, this result is not conclusive and more studies are needed.

Conclusion: The results could pave the way for the use of chABC embedded in the scaffold for the treatment of SCI and show that this method of administration is superior to chABC injection alone.

Keywords: Hydrogel; Scaffold; Spinal cord injury: animal model; chABC.

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Conflict of interest statement

MRH states the possible conflicts of interest in Additional file. The other authors declare no conflict of interest.

Figures

Fig. 1
Fig. 1
PRISMA flow diagram for systematic reviews displaying database search details, the number of abstracts, and the full texts included in the study
Fig. 2
Fig. 2
Funnel plot of articles on the effects of chABC embedded in a scaffold on the motor function recovery in animal models of SCI. SMD standardized mean difference
Fig. 3
Fig. 3
Forest plot of the effects of chABC embedded in a scaffold on behavioral tests after SCI compared to non-treated animals. CI confidence interval, SMD standardized mean difference
See this image and copyright information in PMC

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