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. 2023 Jan;17(1):e13064.
doi: 10.1111/irv.13064. Epub 2022 Oct 31.

Factors influencing sensitivity of a rapid influenza diagnostic test in a community-based population of kindergarten through 12th-grade students: Wisconsin 2015-2020

Cristalyne Bell  1 , Jennifer Birstler  2 , Maureen D Goss  1 , Emily Temte  1 , Shari Barlow  1 , Guanhua Chen  2 , Amra Uzicanin  3 , Jonathan Temte  1
Affiliations

Factors influencing sensitivity of a rapid influenza diagnostic test in a community-based population of kindergarten through 12th-grade students: Wisconsin 2015-2020

Cristalyne Bell et al. Influenza Other Respir Viruses. 2023 Jan.

Abstract

Rapid influenza diagnostic tests (RIDTs) have variable sensitivity. In a community-based population of kindergarten through 12th-grade (K-12) students, we assessed factors that may influence RIDT performance using 2368 paired results from Sofia® influenza A + B fluorescent immunoassay and reverse transcription polymerase chain reaction (RT-PCR). RIDT sensitivity and specificity were 76.1% (95% CI: 72.8-79.1) and 97.2% (96.2-97.9), respectively. Factors associated with sensitivity included runny nose (OR = 3.0, p < 0.001), nasal congestion (1.59, p = 0.045), days from symptom onset (per day; 0.75; p < 0.001), myalgia (0.61; p = 0.014), age (per 5 years; 0.55; p = 0.001), and detection of another virus (0.50; p = 0.043). Understanding these factors can aid in interpreting negative results.

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Conflict of interest statement

Dr. Jonathan Temte has received past research funding from Quidel Corporation. Quidel provided in‐kind Sofia® tests for ORCHARDS. Quidel did not direct or exert any influence over study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Figures

FIGURE 1
FIGURE 1
Odds ratios with 95% confidence intervals for factors associated with rapid influenza diagnostic test performance. RVP, respiratory viral panel; ILI, influenza‐like illness
FIGURE 2
FIGURE 2
Sensitivity estimates with 95th percentile Agresti‐Coull confidence limits for Sofia® influenza A + B FIA based on days from symptom onset. The number of rapid influenza positive results are shown over the number of RT‐PCR positive results for each day.
See this image and copyright information in PMC

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