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. 2023 Jan;50(1):167-172.
doi: 10.1007/s11033-022-07905-0. Epub 2022 Nov 1.

MicroRNAs expression in peripheral blood mononuclear cells of patients with multiple sclerosis propose

Mahsa Abolghasemi  1 , Sepide Ali Ashrafi  2 , Milad Asadi  3 , Dariush Shanehbandi  4 , Saeed Sadigh Etehad  2 , Elham Poursaei  1 , Seyed Aria Nejadghaderi  5 , Sheida Shaafi  6
Affiliations

MicroRNAs expression in peripheral blood mononuclear cells of patients with multiple sclerosis propose

Mahsa Abolghasemi et al. Mol Biol Rep. 2023 Jan.

Abstract

Background: MicroRNAs (miRs) are involved in the autoimmune and neurological diseases, including multiple sclerosis (MS), through modulating post-transcriptional gene regulation. Accumulating evidence indicates that miR-10, miR-24a, miR-124, and miR-21 play an imperative role in MS pathogenesis. Therefore, the current research aimed to analyze the expression of the selected miRNAs for MS in Iranian population.

Methods and results: Blood sample of 75 relapsing-remitting MS (RRMS) patients and 75 healthy individuals suffering no neurodegenerative illness was collected. Subsequently, the isolation of peripheral blood mononuclear cells (PBMCs) was performed by employing Ficoll-Hypaque density gradient method. Afterward, total RNA was extracted and subjected to qRT-PCR analysis. The obtained results evidenced that the relative expression of miR-10 (P = 0.0002), miR-21 (P = 0.0014), and miR-124 (P = 0.0091) significantly decreased in RRMS patients compared to healthy participants. On the contrary, no notable change was observed between the studies groups regarding miR-24a expression levels (P = 0.107). ROC curve analysis estimated an area under the curve (AUC) value equal to 0.75 with P = 0.0006 for miR-10, while it was decreased for miR-21 (AUC = 0.67 and P = 0.0054) and miR-124 (AUC = 0.66 and P = 0.012).

Conclusion: The change in miR-10, miR-124, and miR-21 expression patterns was implied to participate in MS development. Further large scale observational studies are recommended.

Keywords: Multiple sclerosis; Real-time PCR; Relapsing-remitting MS; miR-10; miR-124; miR-21; miR-24a; microRNA.

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References

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