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Review
. 2022 Nov 2;13(1):6530.
doi: 10.1038/s41467-022-33661-7.

Bottom-up assembly of viral replication cycles

Oskar Staufer  1   2   3 , Gösta Gantner  4   5 , Ilia Platzman  6   7   8 , Klaus Tanner  4   5 , Imre Berger  6   9   10 , Joachim P Spatz  6   4   7   8
Affiliations
Review

Bottom-up assembly of viral replication cycles

Oskar Staufer et al. Nat Commun. .

Abstract

Bottom-up synthetic biology provides new means to understand living matter by constructing minimal life-like systems. This principle can also be applied to study infectious diseases. Here we summarize approaches and ethical considerations for the bottom-up assembly of viral replication cycles.

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Conflict of interest statement

I.B. declares shareholding in Halo Therapeutics Ltd, related to this Correspondence. Patents describing materials, material compositions, and formulations related to the present approach have been filed by O.S., I.P., J.P.S., and I.B. The remaining authors declare no competing interests.

Figures

Fig. 1
Fig. 1. Viral replication cycle.
The SARS-CoV-2 infection cycle is depicted in a schematic view, highlighting the sequential and modular nature of the replication process. For each step of the viral replication cycle, viruses have evolved specific molecular modules that are efficiently compressed into single virion particles.
See this image and copyright information in PMC

References

    1. Dreher Y, Jahnke K, Bobkova E, Spatz JP, Göpfrich K. Division and regrowth of phase-separated giant unilamellar vesicles**. Angew. Chem. Int. Ed. 2021;60:10661–10669. doi: 10.1002/anie.202014174. - DOI - PMC - PubMed
    1. Weiss, M. et al. Sequential bottom-up assembly of mechanically stabilized synthetic cells by microfluidics. Nat. Mater.10.1038/nmat5005 (2017). - PubMed
    1. Staufer O, et al. Vesicle induced receptor sequestration: mechanisms behind extracellular vesicle-based protein signaling. Adv. Sci. 2022;9:2200201. doi: 10.1002/advs.202200201. - DOI - PMC - PubMed
    1. Tian L, Li M, Patil AJ, Drinkwater BW, Mann S. Artificial morphogen-mediated differentiation in synthetic protocells. Nat. Commun. 2019;10:3321. doi: 10.1038/s41467-019-11316-4. - DOI - PMC - PubMed
    1. Staufer O, et al. Building a community to engineer synthetic cells and organelles from the bottom-up. eLife. 2021;10:e73556. doi: 10.7554/eLife.73556. - DOI - PMC - PubMed

Publication types