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. 2022 Dec:65:102951.
doi: 10.1016/j.scr.2022.102951. Epub 2022 Oct 19.

Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients caused by heterozygous mutations in the HCN4 gene

Zehra Yildirim  1 , Ana Kojic  1 , Christopher D Yan  1 , Matthew A Wu  1 , Randall Vagelos  2 , Joseph C Wu  3
Affiliations

Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients caused by heterozygous mutations in the HCN4 gene

Zehra Yildirim et al. Stem Cell Res. 2022 Dec.

Abstract

Dilated cardiomyopathy (DCM) is a progressive heart muscle disease that can culminate with heart failure and death. Mutations in several genes can cause DCM, including hyperpolarization-activated cyclic nucleotide-gated channel (HCN4), which has a critical function in the autonomic control of the heart rate. Here, we generated two human induced pluripotent stem cell (iPSC) lines generated from two DCM patients carrying variants in the HCN4 gene (c.2587G > T and c.2846G > A). Both lines display normal karyotype, typical morphology of pluripotent stem cells, and differentiate into all three germ layers in vitro. These lines are valuable resources for studying the pathological mechanisms of DCM.

Keywords: Dilated cardiomyopathy; HCN4; Stem cells; iPSC.

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Conflict of interest statement

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Fig. 1.
Fig. 1.
Characterization of the two DCM lines carrying heterozygous HCN4 gene mutation.
See this image and copyright information in PMC

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