Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2022 Dec;85(6):702-769.
doi: 10.1016/j.jinf.2022.10.036. Epub 2022 Nov 2.

Development of monoclonal antibody-based antigens detection assays for orthopoxvirus and monkeypox virus

Mengjun Li  1 , Yuelin Wang  1 , Chaohui Li  2 , Rui Xu  3 , Jiayin Chen  1 , Junfang Zhang  4 , Minghui Yang  5 , Yushan Jiang  1 , Yuqing Li  1 , Zhujun Zeng  6 , Zhiguang Wu  2 , Wei Zhao  1 , Bo Wu  7 , Chenguang Shen  8
Affiliations
Comment

Development of monoclonal antibody-based antigens detection assays for orthopoxvirus and monkeypox virus

Mengjun Li et al. J Infect. 2022 Dec.
No abstract available

PubMed Disclaimer

Conflict of interest statement

Declaration of Competing Interest The authors declare no competing interests.

Figures

Fig 1
Fig. 1
Purifications, CDR sequences, antigen binding activities and double antibody sandwich antigen or virus detection activities of three monoclonal antibodies against A29L protein of MPXV. (A) SDS-PAGE analysis of purified 3A1, 9F8 and 2D1 antibodies which specific to A29L of MPXV. (B) CDR sequences of 3A1, 9F8 and 2D1 antibodies. (C) Reactivity of 3A1 with MPXV A29L protein in ELISA. (D) Reactivity of 3A1 with camelpox A27L protein in ELISA. (E) Reactivity of 3A1 with taterapox IMV protein in ELISA. (F) Reactivity of 9F8 with MPXV A29L protein in ELISA. (G) Reactivity of 9F8 with camelpox A27L protein in ELISA. (H) Reactivity of 9F8 with taterapox IMV protein in ELISA. (I) Reactivity of 2D1 with MPXV A29L protein in ELISA. (J) Reactivity of 2D1 with camelpox A27L protein in ELISA. (K) Reactivity of 2D1 with taterapox IMV protein in ELISA. (L) Detection of MPXV A29L protein using the 9F8-3A1 sandwich ELISA. (M) Detection of camelpox A27L protein using the 9F8-3A1 sandwich ELISA. (N) Detection of taterapox IMV protein using the 9F8-3A1 sandwich ELISA. (O) Detection of MPXV A29L protein using the 3A1-2D1 sandwich ELISA. (P) Detection of camelpox A27L protein using the 3A1-2D1 sandwich ELISA. (Q) Detection of taterapox IMV protein using the 3A1-2D1 sandwich ELISA. (R) Detection of authentic vaccinia virus using the 9F8-3A1 sandwich ELISA. (S) Detection of vaccinia virus using the 3A1-2D1 sandwich ELISA. (T) The rapid detection assay of a gradient concentration of vaccinia virus, in the format of LFIA, using LFIA test strip and two highly specific monoclonal antibodies against the A29L protein (9F8 for coating and 3A1 for conjugating). (U) The rapid detection assay of a gradient concentration of MPXV A29L protein, in the format of a LFIA, using LFIA test strip and two highly specific monoclonal antibodies against the A29L protein (3A1 for coating and 2D1 for conjugating). NC-Ab, a negative antibody which specific to SARS-CoV-2 generated by our laboratory. NC-Ag, a negative antigen (a receptor binding domain protein of SARS-CoV-2). NC-Virus, an influenza virus strain.
See this image and copyright information in PMC

Comment on

  • CRISPR-Cas12a-based detection of monkeypox virus.
    Sui Y, Xu Q, Liu M, Zuo K, Liu X, Liu J. Sui Y, et al. J Infect. 2022 Dec;85(6):702-769. doi: 10.1016/j.jinf.2022.08.043. Epub 2022 Sep 7. J Infect. 2022. PMID: 36084670 Free PMC article. No abstract available.

References

    1. Sui Y., et al. CRISPR-Cas12a-based detection of monkeypox virus. J Infect. 2022 doi: 10.1016/j.jinf.2022.08.043. - DOI - PMC - PubMed
    1. Petersen E., et al. Human monkeypox: epidemiologic and clinical characteristics, diagnosis, and prevention. Infect Dis Clin N Am. 2019;33:1027–1043. doi: 10.1016/j.idc.2019.03.001. - DOI - PMC - PubMed
    1. Centers for Disease Control and Prevention. 2022 Monkeypox Outbreak Global Map. Available at: https://www.cdc.gov/poxvirus/monkeypox/response/2022/world-map.html. (accessed November 6, 2022).
    1. Reynolds M.G., McCollum A.M., Nguete B., Shongo Lushima R., Petersen B.W. Improving the care and treatment of monkeypox patients in low-resource settings: applying evidence from contemporary biomedical and smallpox biodefense research. Viruses. 2017;9 doi: 10.3390/v9120380. - DOI - PMC - PubMed
    1. Bunge E.M., et al. The changing epidemiology of human monkeypox-A potential threat? A systematic review. PLoS Negl Trop Diss. 2022;16 doi: 10.1371/journal.pntd.0010141. - DOI - PMC - PubMed

Substances