Apparent prevalence of Mycoplasma wenyonii, Candidatus Mycoplasma haemobos, and bovine leukemia virus in Wisconsin and Michigan dairy cattle herds

Abstract

Mycoplasma wenyonii (formerly Eperythrozoon wenyonii) is a hemotrophic, epicellular bacterial parasite of cattle that has been associated with clinical disorders, including hemolytic anemia, decreased milk yield, and peripheral edema. Mycoplasma wenyonii and a related organism, Candidatus Mycoplasma haemobos, have been detected in both ill and apparently healthy cattle, but little is known about their prevalence in US dairy cattle. The objective of this prospective, cross-sectional study was to determine herd-level apparent prevalence of M. wenyonii and C. M. haemobos in dairy cattle located in Wisconsin and Michigan compared with seroprevalence of bovine leukemia virus (BLV) in the same herds. In summer 2018, researchers collected blood samples from 30 lactating cows per herd from randomly recruited farms in selected dairy-intensive counties in each state. During the farm visit, a brief survey was used to collect herd management information. Detection of M. wenyonii and C. M. haemobos were based on PCR testing, and ELISA was used to test for antibodies to BLV. Blood samples were collected from lactating cows located in 64 Wisconsin herds (n = 1,930 samples) and 18 Michigan herds (n = 591 samples). Herd-level apparent prevalence was 100% for both M. wenyonii and C. M. haemobos. Herd-level seroprevalence for BLV was 83 and 100% for Wisconsin and Michigan herds, respectively. Estimated within-herd apparent prevalence of M. wenyonii was 71.7% ± 1.0% (ranging from 23.3 to 93.5%) and for C. M. haemobos was 77.3% ± 1.0% (ranging from 16.7 to 100%). Within-herd prevalence of BLV positive samples was 39.8% ± 1.0% and ranged from 0 to 86.7%. About 22% of cows were concurrently positive for all 3 organisms. Parity and stage of lactation were recorded for 2,317 cows. Prevalence of positive cows for parity groups 1, 2, and ≥3 were 72.0, 73.8, and 67.7% for M. wenyonii; 80.9, 76.8, and 74.9% for C. M. haemobos; and 25.3, 39.7, and 55.5% for BLV, respectively. None or only minor differences in apparent prevalence were observed based on stage of lactation. This is the first report of the prevalence of hemotrophic mycoplasmas in Wisconsin and Michigan dairy herds and indicates that infection with these organisms is endemic. The impact of infection on cattle health and productivity remains unknown, and risk factors associated with infection warrant further study.

Summary:Mycoplasma wenyonii and a related organism, Candidatus Mycoplasma haemobos, have been detected in both ill and apparently healthy cattle, but little is known about their prevalence in US dairy cattle. Blood samples were collected from 1,930 dairy cows on 64 Wisconsin farms and 591 dairy cows in 18 Michigan dairy farms. Samples were tested for Mycoplasma wenyonii and Candidatus Mycoplasma haemobos using whole-blood PCR, and were tested for serum antibodies to bovine leukemia virus using ELISA. This is the first report of the prevalence of hemotrophic mycoplasmas in Wisconsin and Michigan dairy herds and indicates that infection with these organisms is endemic.

Figure 1

Prevalence of positive hemotrophic mycoplasma PCR tests for Mycoplasma wenyonii and Candidatus Mycoplasma haemobos and serum bovine leukemia virus (BLV) antibody tests by parity for dairy cows sampled in Wisconsin (n = 64) and Michigan (n = 18) herds. Differing letters (a–c) denote significant differences among parities within organism; SE denoted by error bars. P-value derived from Bartlett's test of equal variances.

Figure 2

Prevalence of positive hemotrophic mycoplasma PCR tests for Mycoplasma wenyonii and Candidatus Mycoplasma haemobos and serum bovine leukemia virus (BLV) antibody tests by stage of lactation for dairy cows sampled in Wisconsin (n = 64) and Michigan (n = 18) herds. Differing letters (a, b) denote significant differences among stage of lactation groups within organism; SE denoted by error bars. P-value derived from Bartlett's test of equal variances.