Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Oct 29;3(4):101801.
doi: 10.1016/j.xpro.2022.101801. eCollection 2022 Dec 16.

Protocol for laser-induced chronic ocular hypertension and intracameral injection in nonhuman primates

Difang Sun  1 , Bin Wang  2 , Zhenlan Yang  3 , Zongyi Zhan  4 , Jun Li  5 , Yuqing Lan  6
Affiliations

Protocol for laser-induced chronic ocular hypertension and intracameral injection in nonhuman primates

Difang Sun et al. STAR Protoc. .

Abstract

Laser-induced hypertension in nonhuman primates is used to mimic human glaucoma, the leading cause of irreversible blindness. In this protocol, we detail steps for laser-induced ocular hypertension in nonhuman primates by laser photocoagulation of the trabecular meshwork and subsequent intracameral injection. We further describe recording and evaluation of intraocular pressure changes and peripapillary retinal nerve fiber layer thickness. This protocol can assist researchers improve the success rate and repeatability of the procedure and reduce the number of nonhuman primates needed. For complete details on the use and execution of this protocol, please refer to Sun et al. (2022).

Keywords: Health sciences; Model organisms; Neuroscience.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

None
Graphical abstract
Figure 1
Figure 1
Methods of laser-induced COHT model (A) An operation picture of laser photocoagulation in a rhesus monkey. (B) Anterior chamber angle structure of rhesus monkey and positioning of trabecular meshwork under gonioscopy using a smartphone camera. Scale bar 1 mm. (C) A diagram of anterior chamber angle structure in rhesus monkeys. (D) The laser spot location and a bubble generation.
Figure 2
Figure 2
Procedure of intracameral injection (A) Intracameral injection operation. (B) Using antibiotic eye drops to prevent infection. (C) Local disinfection. (D) Needle tip position in intracameral injection. Scale bar 1 mm. (E) Using antibiotic eye ointment after operation.
Figure 3
Figure 3
Experimental images from COHT model in rhesus monkeys (A) Time course of IOP changes in 14 eyes after 2–4 times laser photocoagulation compared to 2 normal eyes. (B) Representative H&E staining images in trabecular meshwork from normal and COHT eyes. Scale bar 100 μm. (C) Representative SD-OCT pictures showed the progression of peripapillary RNFL in a normal eye and a COHT eye. Scale bar 200 μm.
See this image and copyright information in PMC

References

    1. Aptel F., Weinreb R.N., Chiquet C., Mansouri K. 24-h monitoring devices and nyctohemeral rhythms of intraocular pressure. Prog. Retin. Eye Res. 2016;55:108–148. - PubMed
    1. Bastia E., Toris C.B., Brambilla S., Galli C., Almirante N., Bergamini M.V.W., Masini E., Sgambellone S., Unser A.M., Ahmed F., et al. NCX 667, a novel nitric oxide donor, lowers intraocular pressure in rabbits, dogs, and non-human primates and enhances TGFβ2-induced outflow in HTM/HSC constructs. Invest. Ophthalmol. Vis. Sci. 2021;62:17. - PMC - PubMed
    1. Fuwa M., Toris C.B., Fan S., Taniguchi T., Ichikawa M., Odani-Kawabata N., Iwamura R., Yoneda K., Matsugi T., Shams N.K., Zhang J.Z. Effects of a novel selective EP2 receptor agonist, omidenepag isopropyl, on aqueous humor dynamics in laser-induced ocular hypertensive monkeys. J. Ocul. Pharmacol. Ther. 2018;34:531–537. - PubMed
    1. Gloe S., Rothering A., Kiland J.A., McLellan G.J. Validation of the Icare(®) TONOVET plus rebound tonometer in normal rabbit eyes. Exp. Eye Res. 2019;185:107698. - PMC - PubMed
    1. Kelly D.J., Farrell S.M. Physiology and role of intraocular pressure in contemporary anesthesia. Anesth. Analg. 2018;126:1551–1562. - PubMed

Publication types