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. 2022 Nov 8;204(12):693.
doi: 10.1007/s00203-022-03313-x.

Genetic diversity and population structure of Saccharomyces cerevisiae isolated from Turkish sourdough by iPBS-retrotransposons markers

Furkan Aydın  1 , Göksel Özer  2 , Mehtap Alkan  2 , İbrahim Çakır  3
Affiliations

Genetic diversity and population structure of Saccharomyces cerevisiae isolated from Turkish sourdough by iPBS-retrotransposons markers

Furkan Aydın et al. Arch Microbiol. .

Abstract

Molecular DNA markers are valuable tools for analyzing genetic variation among yeast from different populations to reveal the genetically different autochthonous strains. In this study, we employed inter-primer binding site (iPBS) retrotransposon polymorphism to assess the genetic variation and population structure of 96 Saccharomyces cerevisiae isolates from four different regions in Turkey. The nine selected iPBS primers amplified 102 reproducible and scorable bands, of which 95.10% were polymorphic with an average of 10.78 polymorphic fragments per primer. The average polymorphism information content and the resolving power were 0.26-3.58, respectively. Analysis of molecular variance (AMOVA) revealed significant (P < 0.001) genetic differences within populations (88%) and between populations (12%). The unweighted pair group mean with arithmetic (UPGMA) dendrogram grouped 96 S. cerevisiae strains into two main clusters, where the highest probability of the data elucidating the population structure was obtained at ΔK = 2. There was not an obvious genetic discrimination of the populations according to geographical regions on UPGMA, supported by principal coordinate analysis. However, the individuals of the closer provinces in each population were more likely to group together or closely. The results indicate that iPBS polymorphism is a useful tool to reveal the genetically diverse autochthonous S. cerevisiae strains that may be important for the production of sourdough or baked goods.

Keywords: Heterogeneity; Marker; Retrotransposons; Sourdough; Yeast; iPBS.

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Conflict of interest statement

The authors declare that there is no conflict of interest for this research.

Figures

Fig. 1
Fig. 1
Location of sourdough samples from which the S. cerevisiae strains were isolated. Different colors represent different sampling regions. CAR: Central Anatolia region, BSR black sea region, MER mediterranean region, AER aegean region
Fig. 2
Fig. 2
The UPGMA dendrogram of 96 Saccharomyces cerevisiae isolates based on Jaccard’s coefficient
Fig. 3
Fig. 3
Valued amount of K (ΔK) obtained by STRUCTURE HARVESTER using the Evanno method
Fig. 4
Fig. 4
Population structure of the individuals at ΔK = 2 based on the iPBS-retrotransposons data. CAR central Anatolia region, BSR black Sea region, MER mediterranean region, AER aegean region
Fig. 5
Fig. 5
The distribution of Saccharomyces cerevisiae populations according to Principle Coordinate Analysis. CAR central Anatolia region, BSR black Sea region, MER mediterranean region, AER aegean region
See this image and copyright information in PMC

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