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. 2023 Jan;42(1):137-152.
doi: 10.1007/s00299-022-02941-3. Epub 2022 Nov 8.

Overexpression of the GmEXPA1 gene reduces plant susceptibility to Meloidogyne incognita

Marcos Fernando Basso  1   2 , Isabela Tristan Lourenço-Tessutti  1   2 , Clidia Eduarda Moreira-Pinto  1   3 , Reneida Aparecida Godinho Mendes  1   3 , Debora Gonçalves Pereira  1   3 , Adriana Grandis  4 , Leonardo Lima Pepino Macedo  1   2 , Amanda Ferreira Macedo  4 , Ana Cristina Meneses Mendes Gomes  1 , Fabrício Barbosa Monteiro Arraes  1   2 , Roberto Coiti Togawa  1   2 , Marcos Mota do Carmo Costa  1 , Francismar Corrêa Marcelino-Guimaraes  2   5 , Maria Cristina Mattar Silva  1   2 , Eny Iochevet Segal Floh  4 , Marcos Silveira Buckeridge  4 , Janice de Almeida Engler  2   6 , Maria Fatima Grossi-de-Sa  7   8   9
Affiliations

Overexpression of the GmEXPA1 gene reduces plant susceptibility to Meloidogyne incognita

Marcos Fernando Basso et al. Plant Cell Rep. 2023 Jan.

Abstract

The overexpression of the soybean GmEXPA1 gene reduces plant susceptibility to M. incognita by the increase of root lignification. Plant expansins are enzymes that act in a pH-dependent manner in the plant cell wall loosening and are associated with improved tolerance or resistance to abiotic or biotic stresses. Plant-parasitic nematodes (PPN) can alter the expression profile of several expansin genes in infected root cells. Studies have shown that overexpression or downregulation of particular expansin genes can reduce plant susceptibility to PPNs. Root-knot nematodes (RKN) are obligate sedentary endoparasites of the genus Meloidogyne spp. of which M. incognita is one of the most reported species. Herein, using a transcriptome dataset and real-time PCR assays were identified an expansin A gene (GmEXPA1; Glyma.02G109100) that is upregulated in the soybean nematode-resistant genotype PI595099 compared to the susceptible cultivar BRS133 during plant parasitism by M. incognita. To understand the role of the GmEXPA1 gene during the interaction between soybean plant and M. incognita were generated stable A. thaliana and N. tabacum transgenic lines. Remarkably, both A. thaliana and N. tabacum transgenic lines overexpressing the GmEXPA1 gene showed reduced susceptibility to M. incognita. Furthermore, plant growth, biomass accumulation, and seed yield were not affected in these transgenic lines. Interestingly, significant upregulation of the NtACC oxidase and NtEFE26 genes, involved in ethylene biosynthesis, and NtCCR and Nt4CL genes, involved in lignin biosynthesis, was observed in roots of the N. tabacum transgenic lines, which also showed higher lignin content. These data suggested a possible link between GmEXPA1 gene expression and increased lignification of the root cell wall. Therefore, these data support that engineering of the GmEXPA1 gene in soybean offers a powerful biotechnology tool to assist in RKN management.

Keywords: Glycine max; Plant-nematode interaction; RKN management; Root cell wall; Root lignification; Root-knot nematodes.

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