Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Mar;111(3):309-321.
doi: 10.1002/jbm.a.37470. Epub 2022 Nov 9.

Morphofunctional reaction of leukocytes and platelets in in vitro contact with a-C:H:SiOx -coated Ti-6Al-4V substrate

Alexander S Grenadyorov  1 , Andrey A Solovyev  1 , Konstantin V Oskomov  1 , Vjacheslav A Semenov  1 , Maksim O Zhulkov  1   2 , Dmitriy A Sirota  1   2 , Alexander M Chernyavskiy  1   2 , Nataly A Karmadonova  2 , Vladimir V Malashchenko  1   3 , Larisa S Litvinova  3 , Olga G Khaziakhmatova  3 , Natalia D Gazatova  3 , Igor A Khlusov  3   4
Affiliations

Morphofunctional reaction of leukocytes and platelets in in vitro contact with a-C:H:SiOx -coated Ti-6Al-4V substrate

Alexander S Grenadyorov et al. J Biomed Mater Res A. 2023 Mar.

Abstract

The article deals with the plasma-assisted chemical vapor deposition of 0.3-1.4 μm thick a-C:H:SiOx films in a mixture of argon and polyphenylmethylsiloxane vapor onto the Ti-6Al-4V alloy substrate, which is often used as an implant material. The a-C:H:SiOx film structure is studied by the Fourier-transform infrared and Raman spectroscopies. The pull-off adhesion test assesses the adhesive strength of a-C:H:SiOx films, and the ball-on-disk method is employed to measure their wear rate and friction coefficient. According to these studies, a-C:H:SiOx films are highly adhesive to the Ti-6Al-4V substrate, have low (0.056) friction coefficient and wear rate (9.8 × 10-8 mm3 N-1 m-1 ) in phosphate-buffered saline at 40°C. In vitro studies show neither thrombogenicity nor cytotoxicity of the a-C:H:SiOx film for the human blood mononuclear cells (hBMNCs). The in vitro contact between the hBMNC culture and a-C:H:SiOx films 0.8-1.4 μm thick deposited onto Ti-6Al-4V substrates reduces a 24-hour secretion of pro-inflammatory cytokines and chemokines IL-8, IL-17, TNFα, RANTES, and MCP-1. This reduction is more significant when the film thickness is 1.4 μm and implies its potential anti-inflammatory effect and possible application in cardiovascular surgery. The dependence is suggested for the concentration of anti-inflammatory cytokines and chemokines and the a-C:H:SiOx film thickness, which correlates with the surface wettability and electrostatic potential. The article discusses the possible applications of the anti-inflammatory effect and low thrombogenicity of a-C:H:SiOx films in cardiovascular surgery.

Keywords: a-C:H:SiOx film; biocompatibility; human blood mononuclear cells; in vitro cytotoxicity; secretory activity; thrombogenicity.

PubMed Disclaimer

References

REFERENCES

    1. Gepreel M, Niinomi M. Biocompatibility of Ti-alloys for long-term implantation. J Mech Behav Biomed Mater. 2013;20:407-415.
    1. Tian XJ, Zhang SQ, Li A, Wang HM. Effect of annealing temperature on the notch impact toughness of a laser melting deposited titanium alloy Ti-4Al-1.5Mn. Mater Sci Eng A. 2010;527:1821-1827.
    1. Kim JG, Lee KR, Yang SJ. Wear-corrosion performance of Si-DLC coatings on Ti-6Al-4V substrate. J Biomed Mater Res A. 2008;86(1):41-47.
    1. Ding HH, Fridrici V, Guillonneau G, et al. Investigation on mechanical properties of tribofilm formed on Ti-6Al-4V surface sliding against a DLC coating by nano-indentation and micro-pillar compression techniques. Wear. 2019;432-433:202954.
    1. Ding HH, Fridrici V, Geringer J, Fontaine J, Kapsa P. Influence of diamond-like carbon coatings and roughness on fretting behaviors of Ti-6Al-4V for neck adapter-femoral stem contact. Wear. 2018;406-407:53-67.

Publication types

LinkOut - more resources