Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Sep;14(9):1521-9.

[Molecular cloning of the structural genes of the Escherichia coli deo-operon in plasmid RSF2124]

[Article in Russian]
A S Mironov, M N Rozinov, Iu I Kozlov, V V Sukhodolets, B A Rebentish
  • PMID: 363516

[Molecular cloning of the structural genes of the Escherichia coli deo-operon in plasmid RSF2124]

[Article in Russian]
A S Mironov et al. Genetika. 1978 Sep.

Abstract

A specialized phage lambda ddeo carrying the deo operon of Escherichia coli is analyzed by exposing the DNA to the specific restriction endonucleases EcoRI and BamHI. Using the lambda ddeo DNA fragment, obtained by digestion with BamHI and plasmid RSF2124 as a vehicle, the hybrid plasmid pAM1 carrying all the genes of the deo operon is constructed and cloned in E. coli cells. It is shown that the activity of thymidine phosphorylase in the strain AM061, which contains hybrid plasmid pAM1 is 30-fold greater than that in strains of E. coli with chromosomal localization of the deo operon.

PubMed Disclaimer

Substances