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. 2023 Feb;64(1):81-87.
doi: 10.1007/s13353-022-00736-6. Epub 2022 Nov 10.

No dynamic changes in the expression of genes related to the epigenetic mechanism during acute exercise

Witold Józef Światowy  1 , Jacek Zieliński  2 , Maria Aleksandra Osielska  3 , Krzysztof Kusy  2 , Dariusz Wieliński  4 , Andrzej Pławski  5   6 , Paweł Piotr Jagodziński  7
Affiliations

No dynamic changes in the expression of genes related to the epigenetic mechanism during acute exercise

Witold Józef Światowy et al. J Appl Genet. 2023 Feb.

Abstract

Physical exercise results in structural remodeling in tissues and modifies cellular metabolism. Changes in gene expression lie at the root of these adaptations. Epigenetic changes are one of the factors responsible for such exercise-related alterations. One-hour acute exercise will change DNMT1, HDAC1, and JHDM1D transcriptions in PBMC. This study examined changes in the expression of genes responsible for epigenetic modifications (HDAC1, DNMT1, and JHDM1D) during and after an incremental exercise test on a treadmill and a 30-min recovery. Blood samples from 9 highly trained triathletes were tested. Examination of the transcripts showed no significant changes. Correlations between transcript results and biochemical indices revealed a significant (p = 0.007) relationship between JHDM1D mRNA and the number of monocytes at peak exercise intensity (exhaustion), while there was no significant (p = 0.053) correlation at rest. There are no rapid changes in the mRNA levels of the genes studied in blood cells in competitive athletes during acute exercise and recovery. Due to the small group of subjects studied, more extensive research is needed to verify correlations between transcription and biochemical variables.

Keywords: Acute exercise; DNA methylation; DNMT1; Epigenetic; HDAC1; JHDM1D.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
The graph shows the relative mRNA level of the JHDM1D, DNMT1, and HDAC1 genes in PBMC of the tested athletes in relation to the duration of the exercise test and recovery. The value of the transcription rate was determined relative to the reference genes and presented as the logarithm of the multiplicity of the cDNA obtained after the qPCR reaction
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