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. 2022 Oct 24:13:1011001.
doi: 10.3389/fpls.2022.1011001. eCollection 2022.

Fungal isolates influence the quality of Peucedanum praeruptorum Dunn

Li Liu  1   2 , Xuejun Wang  1   2 , Shaotong Chen  3 , Dong Liu  1   2 , Cheng Song  1   2 , Shanyong Yi  1   2 , Fucheng Zhu  1   2 , Wei Wang  1   2 , Fang Wang  1   2 , Guanglin Wang  4 , Xiangwen Song  1   2 , Bin Jia  1   2 , Cunwu Chen  1   2 , Huasheng Peng  5 , Lanping Guo  6 , Bangxing Han  1   2
Affiliations

Fungal isolates influence the quality of Peucedanum praeruptorum Dunn

Li Liu et al. Front Plant Sci. .

Abstract

The symbiotic relationship between beneficial microorganisms and plants plays a vital role in natural and agricultural ecosystems. Although Peucedanum praeruptorum Dunn is widely distributed, its development is greatly limited by early bolting. The reason for early bolting in P. praeruptorum remains poorly characterized. We focus on the plant related microorganisms, including endophytes and rhizosphere microorganisms, by combining the traditional isolation and culture method with metagenomic sequencing technology. We found that the OTUs of endophytes and rhizosphere microorganisms showed a positive correlation in the whole growth stage of P. praeruptorum. Meanwhile, the community diversity of endophytic and rhizosphere fungi showed an opposite change trend, and bacteria showed a similar change trend. Besides, the microbial communities differed during the pre- and post-bolting stages of P. praeruptorum. Beneficial bacterial taxa, such as Pseudomonas and Burkholderia, and fungal taxa, such as Didymella and Fusarium, were abundant in the roots in the pre-bolting stage. Further, a strain belonging to Didymella was obtained by traditional culture and was found to contain praeruptorin A, praeruptorin B, praeruptorin E. In addition, we showed that the fungus could affect its effective components when it was inoculated into P. praeruptorum. This work provided a research reference for the similar biological characteristics of perennial one-time flowering plants, such as Saposhnikovia divaricate, Angelica sinensis and Angelica dahurica.

Keywords: Peucedanum praeruptorum Dunn; endophytes; field experiment; rhizosphere microorganisms; secondary metabolites.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Co-occurrence networks of endophytes and rhizosphere microbial community. (A) endophytes and rhizosphere microbial community in the whole growth stage; (B) endophytic bacteria and fungi in pre-bolting stage; (C) endophytic bacteria and fungi in post-bolting stage; (D) rhizosphere bacteria and fungi in pre-bolting stage; and (E) rhizosphere bacteria and fungi in post-bolting stage.
Figure 2
Figure 2
Shannon index line chart of Qianhu in the whole growth stage. (A) Fungi and (B) Bacterial.
Figure 3
Figure 3
Differences of Qianhu in pre- and post-bolting periods. (A) Number of OTUs shared among different Qianhu samples. The number in the shaded overlap area indicated the total number of OTUs shared between sample types. (B) Sample diversity measurements of endophytic and rhizosphere samples. Species diversity was estimated according to the Shannon index. (C) Species community richness was estimated according to the ACE index. (D) PCoA analysis between roots and soil samples based on weighted unifrac distance. Bacteria can be found on the left and fungi on the right.
Figure 4
Figure 4
Taxonomic composed of bacterial and fungal microbiomes. (A) Bacterial community composition in the pre-and post-bolting stages of Qianhu; (B) Fungal community composition; (C) Comparison of bacterial communities that decreased after bolting as screened by LEfSe analysis; (D) Comparison of fungal communities.
Figure 5
Figure 5
Phylogenetic tree of endophytic fungi in Qianhu. Each color corresponds to a class.
Figure 6
Figure 6
Analyzed of different fungal abundances in different treatment groups.
See this image and copyright information in PMC

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