Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Nov 3;13(11):1017.
doi: 10.3390/insects13111017.

Transcriptome Analysis Revealed Genes Related to γ-Irradiation Induced Emergence Failure in Third-Instar Larvae of Bactrocera dorsalis

Chao Sun  1 , Samina Shabbir  2   3 , Wenxiang Wang  4 , Yan Gao  2   3 , Cuicui Ge  5 , Qingsheng Lin  2   3
Affiliations

Transcriptome Analysis Revealed Genes Related to γ-Irradiation Induced Emergence Failure in Third-Instar Larvae of Bactrocera dorsalis

Chao Sun et al. Insects. .

Abstract

The oriental fruit fly is a polyphagous and highly invasive economically important pest in the world. We proposed the hypothesis that radiation treatment influence RNA expression in the larvae and leads to emergence failure. Therefore, transcriptome analyses of third-instar larvae of B. dorsalis ionizing, irradiated with 60Co-γ at 116Gy, were conducted and compared with the controls; a total of 608 DEGs were identified, including 348 up-regulated genes and 260 down-regulated ones. In addition, 130 SNPs in 125 unigenes were identified. For the DEGs, the most significantly enriched GO item was hemolymph coagulation, and some of the enriched pathways were involved in digestive processes. The subsequent validation experiment confirmed the differential expression of six genes, including sqd, ENPEP, Jhe, mth, Notch, and Ugt. Additionally, the 3401:G->A SNP in the Notch gene was also successfully validated. According to previous research, this was the first comparative transcriptome study to discover the candidate genes involved in insect molt to pupae. These results not only deepen our understanding of the emerging mechanism of B. dorsalis but also provide new insights into the research of biomarkers for quarantine insect treatment with the appropriate dose of radiation.

Keywords: biomarker; invasive; ionizing irradiation; oriental fruit fly; quarantine.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Species distribution of the BLASTX matches (cutoff value E < 10−5) of the transcriptome unigenes.
Figure 2
Figure 2
Classification of the clusters of orthologous groups (COG) for the transcriptome.
Figure 3
Figure 3
Classification of the gene ontology (GO) for the transcriptome.
Figure 4
Figure 4
qRT-PCR confirmation of the differentially expressed genes between irradiated samples and controls.
Figure 5
Figure 5
Sanger sequencing results for the SNP identified in the Notch gene.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Mutamiswa R., Nyamukondiwa C., Chikowore G., Chidawanyika F. Overview of oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) in Africa: From invasion, bio-ecology to sustainable management. Crop Prot. 2021;141:105492. doi: 10.1016/j.cropro.2020.105492. - DOI
    1. Lux S.A., Copeland R.S., White I.M., Manrakhan A., Billah M.K. A new invasive fruit fly species from the Bactrocera dorsalis (Hendel) group detected in East Africa. Int. J. Trop. Insect Sci. 2003;23:355–361. doi: 10.1017/S174275840001242X. - DOI
    1. Leblanc L., Vueti E.T., Drew R.A., Allwood A.J. Host Plant Records for Fruit Flies (Diptera: Tephritidae: Dacini) in the Pacific Islands. Proc. Hawaiian Entomol. Soc. 2012;44:11–53.
    1. Allwood A.J., Chinajariyawong A., Kritsaneepaiboon S., Drew R.A.I., Hamacek E.L., Hancock D.L., Hengsawad C., Jipanin J.C., Jirasurat M., Krong C.K., et al. Host plant records for fruit flies (Diptera: Tephritidae) in Southeast Asia. Raffles Bull. Zool. 1999;47:1–92.
    1. Fletcher B.S. The Biology of Dacine Fruit Flies. Annu. Rev. Entomol. 1987;32:115–144. doi: 10.1146/annurev.en.32.010187.000555. - DOI

LinkOut - more resources