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. 2023:2598:115-121.
doi: 10.1007/978-1-0716-2839-3_9.

The Dimethylmethylene Blue Assay (DMMB) for the Quantification of Sulfated Glycosaminoglycans

Yann D Ladner  1   2 , Mauro Alini  3 , Angela R Armiento  4
Affiliations

The Dimethylmethylene Blue Assay (DMMB) for the Quantification of Sulfated Glycosaminoglycans

Yann D Ladner et al. Methods Mol Biol. 2023.

Abstract

The 1,9-dimethylmethylene blue (DMMB) assay enables the detection of sulfated glycosaminoglycans (sGAGs). This assay can be used to quickly quantify the sGAG content in a large number of samples using spectrophotometry. While this widespread assay appears straightforward, there are certain pitfalls that need to be considered.

Keywords: Cartilage; DMMB; Dimethylmethylene blue assay; Sulfated glycosaminoglycans; sGAG.

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