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. 2023 Jan;415(3):447-456.
doi: 10.1007/s00216-022-04425-5. Epub 2022 Nov 11.

An ultrasensitive electrochemical immunosensor for carcinoembryonic antigen detection based on two-dimensional PtPd/Cu-TCPP(Fe) nanocomposites

Jie Liu  1 , Zixuan Zhang  1 , Ying Li  1 , Jie Dong  1 , Chaorui Li  2
Affiliations

An ultrasensitive electrochemical immunosensor for carcinoembryonic antigen detection based on two-dimensional PtPd/Cu-TCPP(Fe) nanocomposites

Jie Liu et al. Anal Bioanal Chem. 2023 Jan.

Abstract

Establishing an effective signal amplification strategy is the key to achieving sensitive detection of analytes by electrochemical immunoassay. In this work, a novel sandwich-type electrochemical immunosensor with dual-signal amplification was successfully constructed using PtPd/Cu-TCPP(Fe) as the sensing platform and mesoporous silicon dioxide as the signal amplifier. Firstly, two-dimensional wrinkled Cu-TCPP(Fe) nanomaterials loaded with PtPd nanoparticles have strong affinity for the immobilization of capture antibodies and can generate excellent electrochemical signals. Meanwhile, the mesoporous silicon dioxide with large steric hindrance was used as signal label to further improve the sensitivity of the immunosensor by increasing the difference of the current response signal. Under optimal experimental conditions, the electrochemical immunosensor exhibited a wide linear detection range from 0.1 pg/mL to 1.0 μg/mL, with a detection limit as low as 0.166 fg/mL. The experimental results showed that the constructed immunosensor has a great application prospect in clinical biomarker detection.

Keywords: Carcinoembryonic antigen; PtPd/Cu-TCPP(Fe) nanocomposites; Sandwich-type electrochemical immunosensor; Silicon dioxide tag antibody.

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