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. 2022 Oct 25;11(11):1468.
doi: 10.3390/antibiotics11111468.

Untargeted Metabolomics Used to Describe the Chemical Composition, Antioxidant and Antimicrobial Effects of Extracts from Pleurotus spp. Mycelium Grown in Different Culture Media

Giancarlo Angeles Flores  1 , Carolina Elena Girometta  2 , Gaia Cusumano  1 , Paola Angelini  1 , Bruno Tirillini  3 , Federica Ianni  4 , Francesca Blasi  4 , Lina Cossignani  4   5 , Roberto Maria Pellegrino  1 , Carla Emiliani  1 , Roberto Venanzoni  1 , Giuseppe Venturella  6 , Pasqualina Colasuonno  6 , Fortunato Cirlincione  6 , Maria Letizia Gargano  7 , Gokhan Zengin  8 , Alessandra Acquaviva  9 , Simonetta Cristina Di Simone  9 , Giustino Orlando  9 , Luigi Menghini  9   9 , Claudio Ferrante  9
Affiliations

Untargeted Metabolomics Used to Describe the Chemical Composition, Antioxidant and Antimicrobial Effects of Extracts from Pleurotus spp. Mycelium Grown in Different Culture Media

Giancarlo Angeles Flores et al. Antibiotics (Basel). .

Abstract

Pleurotus species isolated in vitro were studied to determine the effect of different media on their production of secondary metabolites, antimicrobial, and antioxidant activity. The different metabolites among Pleurotus samples covered a total of 58 pathways. Comparisons were made between the metabolic profiles of Pleurotus spp. mycelia grown in two substrates: Potato-dextrose-agar-PDA, used as control (S1), and PDA enriched with 0.5 % of wheat straw (S2). The main finding was that the metabolic pathways are strongly influenced by the chemical composition of the growth substrate. The antibacterial effects were particularly evident against Escherichia coli, whereas Arthroderma curreyi (CCF 5207) and Trichophyton rubrum (CCF 4933) were the dermatophytes more sensitive to the mushroom extracts. The present study supports more in-depth investigations, aimed at evaluating the influence of growth substrate on Pleurotus spp. antimicrobial and antioxidant properties.

Keywords: Pleurotus species; antimicrobial effect; metabolomics; phenolic compounds.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Comparative investigation for exploring the effect of different substrates on the metabolic profile was made between Pleurotus spp. mycelia grown in substrates S2. Substrate S1 was taken as a reference and data were calculated as mean differences compared to S1 (calibrator of the relative quantification). In the figure, red indicates a higher probability of metabolic pathway activation, whereas blue suggests a minor one.
Figure 2
Figure 2
Mummichog Pathway Activity Profile: Comparison of metabolic networks present in samples in both polarities.
Figure 2
Figure 2
Mummichog Pathway Activity Profile: Comparison of metabolic networks present in samples in both polarities.
Figure 2
Figure 2
Mummichog Pathway Activity Profile: Comparison of metabolic networks present in samples in both polarities.
Figure 2
Figure 2
Mummichog Pathway Activity Profile: Comparison of metabolic networks present in samples in both polarities.
Figure 2
Figure 2
Mummichog Pathway Activity Profile: Comparison of metabolic networks present in samples in both polarities.
Figure 3
Figure 3
Cumulative distribution of the most abundant phytochemicals in the tested extracts.
See this image and copyright information in PMC

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