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. 2022 Oct 27;12(21):2956.
doi: 10.3390/ani12212956.

Transcriptome Sequencing Reveals Pathways Related to Proliferation and Differentiation of Shitou Goose Myoblasts

Jiahui Chen  1   2 , Shuai Zhang  1   2 , Genghua Chen  1   2 , Xianqi Deng  1   2 , Danlu Zhang  1   2 , Huaqiang Wen  1   2 , Yunqian Yin  1   2 , Zetong Lin  1   2 , Xiquan Zhang  1   2 , Wen Luo  1   2
Affiliations

Transcriptome Sequencing Reveals Pathways Related to Proliferation and Differentiation of Shitou Goose Myoblasts

Jiahui Chen et al. Animals (Basel). .

Abstract

Chinese Shitou goose is a type of large goose with high meat yield. Understanding the genetic regulation of muscle development in Shitou goose would be beneficial to improve the meat production traits of geese. Muscle development is regulated by genes related to myoblast proliferation and differentiation. In this study, the RNA-seq method was used to construct the mRNA and lncRNA expression profiles of Shitou goose myoblasts and myotubes. A total of 1664 differentially expressed (DE) mRNAs and 244 DE-lncRNAs were identified. The alternative mRNA splicing in proliferation and differentiation stages was also analyzed. Notably, pathways enriched in DE-mRNAs, DE-splicing transcripts, and DE-lncRNAs all point to the Wnt signaling pathway, indicating that the Wnt signaling is a key regulatory pathway of muscle development in Shitou goose. We also constructed the interactive network of DE-lncRNAs and DE-mRNAs and revealed some key genes of lncRNAs regulating the proliferation and differentiation of myoblasts. These results provide new insights for the study of the muscle development of the Shitou goose.

Keywords: RNA-seq; Shitou goose; alternative splicing; long non-coding RNAs; mRNA.

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Conflict of interest statement

All authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Shitou goose myoblasts isolation and transcriptome sequencing. (A) The state of proliferation and differentiation of Shitou goose myoblasts; (B) box diagram of transcriptome expression of each sample; (C) the volcano plot showing DEGs between GM and DM.
Figure 2
Figure 2
A functional analysis of DEGs between Shitou goose myoblasts and myotubes. (A) Gene Ontology enrichment of down-regulated DEGs; (B) gene ontology enrichment of up-regulated DEGs; (C) the significantly enriched KEGG pathways of down-regulated DEGs; (D) the significantly enriched KEGG pathways of up-regulated DEGs.
Figure 3
Figure 3
A gene set enrichment analysis of DEGs between Shitou goose myoblasts and myotubes. (A) Significantly enriched Gene Ontology terms by GSEA analysis; (B) significantly enriched KEGG pathways by GSEA analysis; (C) qPCR validation of some DEGs involved in muscle development; (D) RNA-seq results of some DEGs involved in muscle development. The data in C are mean ± S.E.M. with three replicates per group. One-sample t-test was used to assess the difference between the two groups. * p < 0.05; ** p < 0.01.
Figure 4
Figure 4
A differential splicing analysis of mRNA during goose myoblast differentiation. (A) The proportion of various types of DSGs. SE: Skipped Exon. RI: Retained Intron. MXE: Mutually Exclusive Exons. A5SS: Alternative 5′ Splice Site. A3SS: Alternative 3′ Splice Site; (B) KEGG enrichment analysis of DSGs between proliferative and differentiated myoblasts; (C) GO enrichment analysis of DSGs; (D) Venn diagram of DEGs & DSGs.
Figure 5
Figure 5
Differential expression analysis of LncRNA during Shitou goose myoblasts differentiation. (A) Heat map of DE-lncRNA. The heatmap was constructed using Log10 (FPKM) values.; (B) enriched KEGG pathway of DE-lncRNAs; (C) GO enrichment of DE-lncRNAs.
Figure 6
Figure 6
A DE-lncRNA-DEGs interaction network during goose myogenesis.
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References

    1. Henchion M., Moloney A., Hyland J., Zimmermann J., McCarthy S. Review: Trends for meat, milk and egg consumption for the next decades and the role played by livestock systems in the global production of proteins. Animal. 2021;15:100287. doi: 10.1016/j.animal.2021.100287. - DOI - PubMed
    1. Luo W., Lin Z., Chen J., Chen G., Zhang S., Liu M., Li H., He D., Liang S., Luo Q., et al. TMEM182 interacts with integrin beta 1 and regulates myoblast differentiation and muscle regeneration. J. Cachex. Sarcopenia Muscle. 2021;12:1704–1723. doi: 10.1002/jcsm.12767. - DOI - PMC - PubMed
    1. Braun T., Gautel M. Transcriptional mechanisms regulating skeletal muscle differentiation, growth and homeostasis. Nat. Rev. Mol. Cell Biol. 2011;12:349–361. doi: 10.1038/nrm3118. - DOI - PubMed
    1. Wang S., Jin J., Xu Z., Zuo B. Functions and Regulatory Mechanisms of lncRNAs in Skeletal Myogenesis, Muscle Disease and Meat Production. Cells. 2019;8:1107. doi: 10.3390/cells8091107. - DOI - PMC - PubMed
    1. Ye C., Zhong R. Study on the growth model of lion headed geese. J. Shihezi Univ. (Nat. Sci. Ed.) 2005;7:694–697.