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. 2022 Oct 24;27(21):7182.
doi: 10.3390/molecules27217182.

Antibacterial Activity of Dental Composite with Ciprofloxacin Loaded Silver Nanoparticles

Wafa Arif  1   2 , Nosheen Fatima Rana  1   2 , Iqra Saleem  1   2 , Tahreem Tanweer  1   2 , Muhammad Jawad Khan  3 , Sohad Abdulkaleg Alshareef  4 , Huda M Sheikh  5 , Fatima S Alaryani  5 , Manal Othman Al-Kattan  5 , Hanan Ali Alatawi  6 , Farid Menaa  7 , Aroosa Younis Nadeem  1
Affiliations

Antibacterial Activity of Dental Composite with Ciprofloxacin Loaded Silver Nanoparticles

Wafa Arif et al. Molecules. .

Abstract

Resin composites have been widely used in dental restoration. However, polymerization shrinkage and resultant bacterial microleakage are major limitations that may lead to secondary caries. To overcome this, a new type of antibacterial resin composite containing ciprofloxacin-loaded silver nanoparticles (CIP-AgNPs) were synthesized. The chemical reduction approach successfully produced CIP-AgNPs, as demonstrated by FTIR, zeta potential, scanning electron microscopy, and ultraviolet-visible (UV-vis) spectroscopy. CIP-AgNPs were added to resin composites and the antibacterial activity of the dental composite discs were realized against Enterococcus faecalis, Streptococcus mutans, and the Saliva microcosm. The biocompatibility of modified resin composites was assessed and mechanical testing of modified dental composites was also performed. The results indicated that the antibacterial activity and compressive strength of resin composites containing CIP-AgNPs were enhanced compared to the control group. They were also biocompatible when compared to resin composites containing AgNPs. In short, these results established strong ground application for CIP-AgNP-modified dental composite resins.

Keywords: antibacterial activity; ciprofloxacin; dental composite; nanoparticles.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
UV- Spectrograph of AgNPs & CIP-AgNPs.
Figure 2
Figure 2
FTIR Spectra of CIP, AgNPs and CIP-AgNPs.
Figure 3
Figure 3
(a) SEM image of AgNPs; (b) SEM image of CIP-AgNPs.
Figure 4
Figure 4
(a) Zeta Potential of AgNPs; (b) zeta Potential of CIP-AgNPs.
Figure 5
Figure 5
E. faecalis growth of unmodified composite discs (a), composite discs with 1% AgNPs (b), and composite discs with 1% CIP-AgNPs (c).
Figure 6
Figure 6
S. mutans growth of unmodified composite discs (a), composite discs with 1% AgNPs (b), and composite discs with 1% CIP-AgNPs (c).
Figure 7
Figure 7
Saliva microcosm bacterial growth of unmodified composite discs (a), composite discs with 1% AgNPs (b) and composite discs with 1% CIP-AgNPs (c).
Figure 8
Figure 8
CFU/mL of (a) E. faecalis, (b) S. mutans, (c) Saliva microcosm model, antibacterial effect of modified composite resin discs (p < 0.05). **** p < 0.0001.
Figure 9
Figure 9
Hemolytic activity of experimental composite discs.
Figure 10
Figure 10
Compressive strength of experimental composite discs.
See this image and copyright information in PMC

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