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. 2022 Oct 31;27(21):7390.
doi: 10.3390/molecules27217390.

Phenylethanoid and Phenylmethanoid Glycosides from the Leaves of Ligustrum robustum and Their Bioactivities

Shi-Hui Lu  1 , Hao-Jiang Zuo  2 , Jing Huang  3 , Ran Chen  4 , Jia-Ping Pan  1 , Xiu-Xia Li  5
Affiliations

Phenylethanoid and Phenylmethanoid Glycosides from the Leaves of Ligustrum robustum and Their Bioactivities

Shi-Hui Lu et al. Molecules. .

Abstract

The phytochemical study on the leaves of Ligustrum robustum, which have been used as Ku-Ding-Cha, led to the isolation and identification of three new phenylethanoid glycosides and three new phenylmethanoid glycosides, named ligurobustosides R1 (1b), R2-3 (2), R4 (3), S1 (4b), S2 (5), and S3 (6), and five reported phenylethanoid glycosides (7-11). In the bioactivity test, (Z)-osmanthuside B6 (11) displayed strong fatty acid synthase (FAS) inhibitory activity (IC50: 4.55 ± 0.35 μM) as the positive control orlistat (IC50: 4.46 ± 0.13 μM), while ligurobustosides R4 (3) and S2 (5), ligupurpuroside B (7), cis-ligupurpuroside B (8), ligurobustoside N (9), osmanthuside D (10), and (Z)-osmanthuside B6 (11) showed stronger ABTS radical scavenging activity (IC50: 2.68 ± 0.05~4.86 ± 0.06 μM) than the positive control L-(+)-ascorbic acid (IC50: 10.06 ± 0.19 μM). This research provided a theoretical basis for the leaves of L. robustum as a tea with function in treating obesity and diabetes.

Keywords: FAS; Ligustrum robustum; anti-obesity; antioxidant; hypoglycemic; phenylethanoid glycoside; phenylmethanoid glycosides; α-glucosidase.

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Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

Figures

Figure 1
Figure 1
Structures of compounds 1–11 from the leaves of L. robustum.
Figure 2
Figure 2
Key HMBC correlations of compounds 1–6.
See this image and copyright information in PMC

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