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. 2022 Nov 2;11(21):2958.
doi: 10.3390/plants11212958.

Effects of Chilling Treatment on Baicalin, Baicalein, and Wogonin Biosynthesis in Scutellaria baicalensis Plantlets

Hyeon Ji Yeo  1 , Chang Ha Park  2 , Jae Kwang Kim  3 , Ramaraj Sathasivam  4 , Jae Cheol Jeong  1 , Cha Young Kim  1 , Sang Un Park  4   5
Affiliations

Effects of Chilling Treatment on Baicalin, Baicalein, and Wogonin Biosynthesis in Scutellaria baicalensis Plantlets

Hyeon Ji Yeo et al. Plants (Basel). .

Abstract

When plants are exposed to stressful conditions, they modulate their nutrient balance by regulating their primary and secondary metabolisms to adapt. In this study, changes in primary and secondary metabolites elicited by chilling stress treatment and the effects of treatment duration were examined in roots of Scutellaria baicalensis (S. baicalensis) plantlets. The concentrations of most sugars (maltose, glucose, sucrose, and fructose) and of several amino acids (proline and GABA), which are crucial regarding plant defense mechanisms, increased with increasing duration of chilling stress. Furthermore, salicylic acid levels increased after two-day chilling treatments, which may enhance plant tolerance to cold temperatures. The concentrations of flavones (baicalin, baicalein, and wogonin) increased during chilling stress, and those of phenolic acids (ferulic acid and sinapic acid) increased after two-day chilling treatments. The concentrations of these flavones were positively correlated with sucrose levels which acted as energy sources.

Keywords: Scutellaria baicalensis; chilling treatment; flavones; hydrophilic metabolites.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Heatmap representing differences in relative metabolite concentrations in roots of S. baicalensis plantlets treated with chilling. Increase and decrease in the contents of metabolites are indicated by red and blue coloration, respectively.
Figure 2
Figure 2
Scores (A) and loading (B) plots of the PCA model using metabolites from the roots of S. baicalensis plantlets treated with chilling. RT, room temperature (25 °C); ferulate, ferulic acid; succinate, succinic acid; salicylate, salicylic acid; malate, malic acid.
Figure 3
Figure 3
Correlation matrix of metabolites in the roots of S. baicalensis plantlets treated with chilling.
See this image and copyright information in PMC

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