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. 2023 Mar;101(3):740-748.
doi: 10.1111/cbdd.14177. Epub 2022 Nov 25.

Analysis of bioactive compounds using LC-ESI-MS/MS, cytotoxic, antimicrobial effects, and enzyme activities from Cyclotrichium origanifolium

Necmettin Aktepe  1 , Ayşe Baran  2 , Mehmet Nuri Atalar  3   4 , Mehmet Fırat Baran  5 , Cumali Keskin  5 , Abdullah Taşkin  6 , Ömer Yavuz  7 , İbrahim Demirtaş  4 , Ercan Oğuz  4 , Israt Jahan  8
Affiliations

Analysis of bioactive compounds using LC-ESI-MS/MS, cytotoxic, antimicrobial effects, and enzyme activities from Cyclotrichium origanifolium

Necmettin Aktepe et al. Chem Biol Drug Des. 2023 Mar.

Erratum in

Abstract

Cyclotrichium origanifolium is a medicinal plant belonging to the Lamiaceae family. In this study, phenolic content analysis, antimicrobial effects, and cytotoxic effects of extracts of C. origanifolium were investigated. In the extracts, phenolic compound analysis by the liquid chromatography-electrospray ionization-tandem mass spectrometry method, antimicrobial effect by the minimum inhibition concentration method, and cytotoxic effect on human dermal fibroblasts (HDF), glioblastoma cell (U87), ovarian adenocarcinoma cell (Skov-3), and human colorectal adenocarcinoma cell (CaCo-2) cancer cell lines were investigated. Cytotoxicity analyses were performed by the MTT method. In addition, the GST and AChE enzyme activities of the extracts were also measured. Around 18 compounds were detected in both the methanol and ethanol extract. It was found that the best antimicrobial effect on Gram-negative Pseudomonas aeruginosa was on methanol extract, while the ethanol extract was on Candida albicans fungus (respectively, 2.50 mg/ml, 5.0 μg/ml). A 500 μg/ml of methanol extract has been shown to have cytotoxic activity high effect on HDF cells. GST and AChE activity were found to decrease in a concentration-dependent manner.

Keywords: Cyclotricium origanifolium; antimicrobial; antioxidant; cytotoxic; phenolics.

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