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. 2023:2603:151-161.
doi: 10.1007/978-1-0716-2863-8_12.

Workflow for Quantitative Proteomic Analysis of Intestinal Organoids Using SILAC

Alexis Gonneaud  1 , Claude Asselin  1 , Véronique Giroux  1 , François-Michel Boisvert  2
Affiliations

Workflow for Quantitative Proteomic Analysis of Intestinal Organoids Using SILAC

Alexis Gonneaud et al. Methods Mol Biol. 2023.

Abstract

Stable isotope labeling by amino acids in cell culture (SILAC) is a strategic quantitative mass spectrometry method to analyze multiple protein samples in different conditions simultaneously. In recent years, 3D cell growth culture conditions have been developed to establish intestinal organoids from isolated crypts, which mimic the intestine's cell composition and organization. Organoids, isolated from normal or diseased tissues, can be used to compare cell distribution and differentiation, signaling pathways, and cell responses to pharmacological agents, therapeutic drugs, endogenous or exogenous metabolites, and environmental stresses, among others. Here, we describe the process of generating SILAC organoids from the mouse small intestine.

Keywords: Intestinal organoids; Mass spectrometry; Proteomics; SILAC.

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References

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