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. 2023:2603:187-198.
doi: 10.1007/978-1-0716-2863-8_15.

SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos

Alistair Langlands  1 , Hamze Beati  2 , H- Arno J Müller  3
Affiliations

SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos

Alistair Langlands et al. Methods Mol Biol. 2023.

Abstract

The fruit fly Drosophila melanogaster represents a classic genetic model organism that is amenable to a plethora of comprehensive analyses including proteomics. SILAC-based quantitative proteomics is a powerful method to investigate the translational and posttranslational regulation ongoing in cells, tissues, organs, and whole organisms. Here we describe a protocol for routine SILAC labeling of Drosophila adults within one generation to produce embryos with a labeling efficiency of over 92%. In combination with genetic selection markers, this method permits the quantification of translational and posttranslational changes in embryos mutant for developmental and disease-related genes.

Keywords: Development; Drosophila; Embryo; Posttranslational modification; Proteomics; SILAC.

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