doi: 10.1016/s0378-4347(00)81201-3.
Fluorimetric detection of octopamine in high-performance liquid chromatography and its application to the assay of dopamine beta-monooxygenase in human serum
- PMID: 363735
- DOI: 10.1016/s0378-4347(00)81201-3
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Fluorimetric detection of octopamine in high-performance liquid chromatography and its application to the assay of dopamine beta-monooxygenase in human serum
J Chromatogr.
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Abstract
A high-performance liquid chromatographic procedure is described for the determination of octopamine. The method, which is based on the separation on a microparticulate bonded strong cation-exchange resin and measurement of the native fluorescence, has been applied to give a sensitive assay of dopamine beta-monooxygenase (EC 1.14.17.1) activity in human serum with tyramine as the substrate. The procedure, which has been designed for use with an automatic sampler, has a detection limit of about 50 pmoles of octopamine, and the analysis time is approximately 10 min per sample.
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