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. 2022 Nov;68(5):390-397.
doi: 10.18097/PBMC20226805390.

[Acetylation, methylation, and ubiquitination of proteins in experimental ischemic stroke in mice: a bioinformatics analysis]

[Article in Russian]
V S Skvortsov  1 , Y O Ivanova  1 , A I Voronina  1
Affiliations

[Acetylation, methylation, and ubiquitination of proteins in experimental ischemic stroke in mice: a bioinformatics analysis]

[Article in Russian]
V S Skvortsov et al. Biomed Khim. 2022 Nov.

Abstract
in English, Russian

The experimental results available in the ProteomeXchange database (accession code PXD016538) (Simats et al. (2020) Molecular and Cellular Proteomics, 19(12), 1921-1936) obtained using a comprehensive multi-omics approach were analyzed in mouse blood to identify potential biomarkers of ischemic stroke. Acetylation, methylation, and ubiquitination were considered as post-translational modifications. The analysis of the significance of changes in the level of protein modification was evaluated for ischemic tissue in comparison with tissue undamaged by stroke and control taken from mice after sham operation. At the level of statistically significant differences according to the Mann-Whitney test (p < 0.05), 2 proteins were found (Q02248 and Q8BL66); for additional 7 proteins, the differences were at the level of a statistical trend (p < 0.1). For 7 of 9 selected proteins there are reports in the literature, for their association with cerebral ischemia.

Proanalizirovany dostupnye v baze dannykh ProteomeXchange (accession code PXD016538) éksperimental'nye rezul'taty (Simats et al. (2020) Molecular and Cellular Proteomics, 19(12), 1921-1936), poluchennye pri ispol'zovanii kompleksnogo mul'tiomnogo podkhoda po vyiavleniiu potentsial'nykh biomarkerov ishemicheskogo insul'ta v krovi mysheĭ. V kachestve posttransliatsionnykh modifikatsiĭ rassmotreny atsetilirovanie, metilirovanie i ubikvitinirovanie. Analiz dostovernosti izmeneniia urovnia modifikatsii belkov otsenivali dlia ishemicheskoĭ tkani v sravnenii s nepovrezhdennoĭ insul'tom tkan'iu i kontrolem, vziatym u mysheĭ posle mnimoĭ operatsii (sham operation). Pri urovne statisticheski znachimykh razlichiĭ po kriteriiu Manna-Uitni (p < 0,05) naĭdeno 2 belka (Q02248 i Q8BL66); dlia eshche 7 belkov razlichiia nakhodiatsia na urovne statisticheskoĭ tendentsii (p < 0,1). Dlia 7 iz naĭdennykh 9 belkov v literature est' dannye o sviazi s ishemieĭ mozga.

Keywords: bioinformatics; ischemic stroke; posttranslational modifications.

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