Super-resolution re-scan second harmonic generation microscopy
- PMID: 36375085
- PMCID: PMC9704717
- DOI: 10.1073/pnas.2214662119
Super-resolution re-scan second harmonic generation microscopy
Abstract
Second harmonic generation microscopy (SHG) is generally acknowledged as a powerful tool for the label-free three-dimensional visualization of tissues and advanced materials, with one of its most popular applications being collagen imaging. Despite the great need, progress in super-resolved SHG imaging lags behind the developments reported over the past years in fluorescence-based optical nanoscopy. In this work, we demonstrate super-resolved re-scan SHG, qualitatively and quantitatively showing on collagenous tissues the available resolution advantage over the diffraction limit. We introduce as well super-resolved re-scan two-photon excited fluorescence microscopy, an imaging modality not explored to date.
Keywords: second harmonic generation; super-resolved optical imaging; two-photon excited fluorescence.
Conflict of interest statement
Competing interest statement: E.M.M.M. has relevant ownership of stock in Confocal.nl. B.V. Confocal.nl is owner of the following patents relevant for re-scan microscopy: NL2026002B1, NL2023384B1, NL2020516B1, NL2018134B1.
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