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. 2022 Nov 14;24(6):116.
doi: 10.1208/s12248-022-00770-6.

A Non-radiometric Approach to Determine Tissue Vascular Blood Volume in Biodistribution Studies

Hossein Salimi-Moosavi  1 , Marcus Soto  2
Affiliations

A Non-radiometric Approach to Determine Tissue Vascular Blood Volume in Biodistribution Studies

Hossein Salimi-Moosavi et al. AAPS J. .

Abstract

The aim of this research was to develop a reliable non-radiometric method to measure the residual blood in tissue without the need for perfusion or radiometric measurements in biodistribution studies. It was found that the perfusion method not only was ineffective in removing blood from tissue, but also introduced additional variability in the determination of tissue drug exposure and was not reproducible across studies. In addition, the use of hemoglobin as an endogenous protein and biomarker for tissue blood content was studied and it was found that hemoglobin measurement in tissue was not a reliable and effective approach for determination of residual blood level in tissue. To evaluate an alternative method for addressing the tissue blood level in biodistribution studies, animals were dosed with a Residual Blood Determinant Reagent (RBDR) 5 min prior to tissue harvesting. The level of RBDR, an exogenous protein, was measured in whole blood homogenate and in tissue lysate. Based on the level of the RBDR, the vascular blood volume (VBV) in tissue was calculated and then the tissue exposures were corrected based on the blood volumes. The tissue VBVs measured by the RBDR method were comparable with the literature values obtained by radiometric measurements.

Keywords: Biodistribution; Pharmacokinetics (PK); Residual blood; Tissue blood content; Vascular blood volume.

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References

    1. Wang W, Wang EQ, Balthasar JP. Monoclonal antibody pharmacokinetics and pharmacodynamics. Clinc Pharmaco Therap. 2008;84(5):548–58. - DOI
    1. Vugmeyster Y, Xu X, Theil FP, Khawli LA, Leach MW. Pharmacokinetics and toxicology of therapeutic protein: advance and challenges. World J Biol Chem. 2012;3(4):73–92. - DOI - PubMed - PMC
    1. Tabrizi MA, Tseng CML, Roskos LK. Elimination mechanism of therapeutic monoclonal antibodies. Drug Discov Today. 2006;11(1/2):81–8. - DOI - PubMed
    1. Tabrizi MA, Bornstein GG, Klakamp SL, Drake A, Knight R. Roskos L. Translational strategies for development of monoclonal antibodies from discovery to clinic. Drug Discov Today. 2009;14(5/6): 298–30.
    1. Mould DR, Sweeney KRD. The pharmacokinetics and pharmacodynamics of monoclonal antibodies-mechanistic modeling applied to drug development. Drug Discov Dev. 2007;10(1):84–96.

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