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. 2023:2596:265-289.
doi: 10.1007/978-1-0716-2831-7_19.

Two-CyDye-Based 2D-DIGE Analysis of Aged Human Muscle Biopsy Specimens

Kay Ohlendieck  1
Affiliations

Two-CyDye-Based 2D-DIGE Analysis of Aged Human Muscle Biopsy Specimens

Kay Ohlendieck. Methods Mol Biol. 2023.

Abstract

The gradual loss of skeletal muscle mass during aging and associated decline in contractile strength can result in reduced fitness, frailty, and loss of independence. In order to better understand the molecular and cellular mechanisms that underlie sarcopenia of old age and the frailty syndrome, as well as identify novel therapeutic targets to treat age-related fiber wasting, it is crucial to develop a comprehensive biomarker signature of muscle aging. Fluorescence two-dimensional gel electrophoresis (2D-DIGE) in combination with sensitive mass spectrometry presents an ideal bioanalytical tool for biomarker discovery in biogerontology. This chapter outlines the application of the 2D-DIGE method for the comparative analysis of human biopsy specimens from middle-aged versus senescent individuals using a two-CyDye-based method.

Keywords: Aging; DIGE; Difference gel electrophoresis; Mass spectrometry; Sarcopenia; Senescence; Skeletal muscle; Two-dimensional gel electrophoresis.

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