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. 2022 Nov 17;21(1):341.
doi: 10.1186/s12936-022-04355-8.

Significance of nested PCR testing for the detection of low-density malaria infection amongst febrile patients from the Malaria Elimination Demonstration Project in Mandla, Madhya Pradesh, India

Akansha Singh  1   2 , Mrigendra P Singh  3 , Sneha Bhandari  4 , Harsh Rajvanshi  3   5 , Sekh Nisar  3   6 , Vinay Telasey  3 , Himanshu Jayswar  7 , Ashok K Mishra  1 , Aparup Das  1 , Harpreet Kaur  8 , Altaf A Lal  3   9 , Praveen K Bharti  10
Affiliations

Significance of nested PCR testing for the detection of low-density malaria infection amongst febrile patients from the Malaria Elimination Demonstration Project in Mandla, Madhya Pradesh, India

Akansha Singh et al. Malar J. .

Abstract

Background: Low-density malaria infections (LDMI) are defined as infections that are missed by the rapid diagnostic test (RDT) and/or microscopy which can lead to continued transmission and poses a challenge in malaria elimination efforts. This study was conducted to investigate the prevalence of LDMI in febrile cases using species-specific nested Polymerase Chain Reaction (PCR) tests in the Malaria Elimination Demonstration Project, where routine diagnosis was conducted using RDT.

Methods: Every 10th fever case from a cross-sectional community based fever surveillance was tested with RDT, microscopy and nested PCR. Parasite DNA was isolated from the filter paper using Chelex based method. Molecular diagnosis by nested PCR was performed targeting 18SrRNA gene for Plasmodium species.

Results: The prevalence of malaria was 2.50% (436/17405) diagnosed by PCR, 1.13% (196/17405) by RDT, and 0.68% (118/ 17,405) by microscopy. Amongst 17,405 febrile samples, the prevalence of LDMI was 1.51% (263/17405) (95% CI 1.33-1.70), which were missed by conventional methods. Logistic regression analysis revealed that illness during summer season [OR = 1.90 (p < 0.05)] and cases screened within three days of febrile illness [OR = 5.27 (p < 0.001)] were the statistically significant predictors of LDMI.

Conclusion: The prevalence of malaria among febrile cases using PCR was 2.50% (436/17405) as compared to 1.13% (196/17405) by RDT. Higher number of the LDMI cases were found in subjects with ≤ 3 days mean duration of reported fever, which was statistically significant (p < 0.001). This observation suggests that an early detection of malaria with a more sensitive diagnostic method or repeat testing of the all negative cases may be useful for curtailing malaria transmission. Therefore, malaria elimination programme would benefit from using more sensitive and specific diagnostic methods, such as PCR.

Keywords: Low-density infection; Malaria; P. falciparum; P. vivax; PCR.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Flow chart showing distribution of cases diagnosed by Rapid Diagnostic Test, Microscopy and Polymerase Chain Reaction
Fig. 2
Fig. 2
Venn diagram showing distribution of malaria positive cases found in different diagnostic methods
See this image and copyright information in PMC

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References

    1. WHO . World malaria report 2021. Geneva: World Health Organization; 2021.
    1. WHO . Global technical strategy for malaria 2016–2030. Geneva: World Health Organization; 2015.
    1. Directorate of National Vector Borne Disease Control Programme, Director General of Health Services, Ministry of Health and Family Welfare. Strategic plan for malaria control in india 2012–2017: a five-year strategic plan. New Delhi. http://www.nvbdcp.gov.in/Doc/Strategic-Action-Plan-Malaria-2012-17.
    1. Sharma RK, Thakor H, Saha K, Sonal G, Dhariwal A, Singh N. Malaria situation in India with special reference to tribal areas. Indian J Med Res. 2015;141:537–545. - PMC - PubMed
    1. Bharti PK, Rajvanshi H, Nisar S, Jayswar H, Saha KB, Shukla MM, et al. Demonstration of indigenous malaria elimination through track-test-treat-track (T4) strategy in a Malaria Elimination Demonstration Project in Mandla, Madhya Pradesh. Malar J. 2020;19:339. - PMC - PubMed