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. 2022 Nov 14;67(11):658-662.
doi: 10.51620/0869-2084-2022-67-11-658-662.

Development of a PCR assay for the detection of human herpes virus type 7

S S Mardanly  1 , S G Mardanly  1 , A A Kazakov  2 , V V Demkin  2 , A M Zatevalov  3 , A Yu Mironov  3   4
Affiliations

Development of a PCR assay for the detection of human herpes virus type 7

S S Mardanly et al. Klin Lab Diagn. .

Abstract

A PCR assay has been developed to identify the DNA of the human herpes virus type 7. The search and selection of conserved regions was carried out by comparing the whole genome nucleotide sequences of HHV-7. A fragment duplicated in the HHV-7 genomes was chosen as a target for amplification. The performance of the assay was tested on a synthetic matrix and clinical samples. The developed assay has high sensitivity and specificity and showed good efficiency in detecting HHV-7 DNA in clinical samples.

Keywords: HHV-7; HHV7; PCR; PCR assay; molecular genetic analysis.

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Conflict of interest statement

The authors declare no conflict of interest.

References

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