Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Feb;27(2):691-703.
doi: 10.1007/s00784-022-04787-1. Epub 2022 Nov 18.

Effect of chitosan irrigant solutions on the release of bioactive proteins from root dentin

Sara Quijano-Guauque  1 , Lilia J Bernal-Cepeda  2 , Félix G Delgado  3 , Jaime E Castellanos  2 , Claudia García-Guerrero  4
Affiliations

Effect of chitosan irrigant solutions on the release of bioactive proteins from root dentin

Sara Quijano-Guauque et al. Clin Oral Investig. 2023 Feb.

Abstract

Objective: To identify the effect of two chitosan solutions on the release of root dentin matrix proteins and to describe the chemical changes observed following conditioning with chelating agents.

Materials and methods: The release of dentin sialoprotein (DSP), transforming growth factor-beta 1 (TGF-β1), vascular endothelial growth factor (VEGF), and platelet-derived growth factor-BB (PDGF-BB) with different chelating agents, including ethylenediaminetetraacetic acid (EDTA), chitosan solution (CS), and nanoparticulate chitosan (CSnp), was investigated. DSP was quantified using an enzyme-linked immunosorbent assay (ELISA). TGF-β1, VEGF, and PDGF-BB were quantified using a cytokine bead panel (CBA). Raman spectroscopy was performed to identify surface chemical changes. Statistical analysis was performed using Kruskal-Wallis test with Mann-Whitney-Wilcoxon rank-sum test (p < 0.05).

Results: TGF-β1, VEGF, and DSP solubilized in all irrigants tested. CSnp showed the highest concentration of DSP. PDGF-BB did not exceed the detection limits. Raman spectroscopy revealed a decrease in the phosphate and carbonate peaks, representing the chelating effect of EDTA, CS, and CSnp. Additionally, CSnp showed the greatest preservation of the amide I and III content.

Conclusion: Proteins can be released from dentin via EDTA, CS, and CSnp conditioning. Raman spectroscopic revealed changes in the inorganic content of the root dentin after chelation. Furthermore, use of CSnp facilitated a preservation of the organic content.

Clinical relevance: Chelation allows the release of proteins, justifying the use of chelating agents in regenerative endodontics. The chitosan-dentin matrix interaction also promotes the protection of the organic content as an additional benefit to its protein releasing effect.

Keywords: Chelating agents; Chitosan; Dentin; Endodontics; Extracellular matrix proteins; Regeneration.

PubMed Disclaimer

References

    1. Xie Z, Shen Z, Zhan P, Yang J, Huang Q, Huang S, Chen L, Lin Z (2021) Functional dental pulp regeneration: basic research and clinical translation. Int J Mol Sci 22:8991. https://doi.org/10.3390/ijms22168991 - DOI
    1. Elnawam H, Abdelmougod M, Mobarak A, Hussein M, Aboualmakarem H, Girgis M, El Backly R (2022) Regenerative endodontics and minimally invasive dentistry: intertwining paths crossing over into clinical translation. Front Bioeng Biotechnol 8(10):837639. https://doi.org/10.3389/fbioe.2022.837639 - DOI
    1. Smith JG, Smith AJ, Shelton RM, Cooper PR (2015) Dental pulp cell behavior in biomimetic environments. J Dent Res 94:1552–1559. https://doi.org/10.1177/0022034515599767 - DOI
    1. Huang X, Li Z, Liu A, Liu X, Guo H, Wu M, Yang X, Han B, Xuan K (2021) Microenvironment influences odontogenic mesenchymal stem cells mediated dental pulp regeneration. Front Physiol 12:656588. https://doi.org/10.3389/fphys.2021.656588 - DOI
    1. Jeeruphan T, Jantarat J, Yanpiset K, Suwannapan L, Khewsawai P, Hargreaves KM (2012) Mahidol study 1: comparison of radiographic and survival outcomes of immature teeth treated with either regenerative endodontic or apexification methods: a retrospective study. J Endod 38:1330–1336. https://doi.org/10.1016/j.joen.2012.06.028 - DOI

MeSH terms

LinkOut - more resources