Antioxidant and Anti-Inflammatory Activity of Ethanol Extract Stem of Etlingera rubroloba A.D. Poulsen

Abstract

<b>Background and Objective:</b> <i>Etlingera rubroloba</i> A.D. Poulsen (<i>E. rubroloba</i>) is a plant endemic to South-East Sulawesi which is used empirically by local people as a pain reliever for joints and to increase endurance after typhoid fever. This study aimed to determine the antioxidant and anti-inflammatory activity of the ethanol extract stem of <i>E. rubroloba</i>. <b>Materials and Methods:</b> The stem of the ethanol extract of <i>E. rubroloba</i> with evaluated for antioxidants using the FRAP method and anti-inflammatory activity with the parameter level of Tumor Necrosis Factor-Alpha (TNF-α) <i>in vivo</i> by using 6 groups of rats, namely the normal, the negative (Na-CMC 0.5%), the positive control (Diclofenac sodium) and the ethanol extract group at doses of 200, 300 and 400 mg kg<sup>1</sup> b.wt. <b>Results:</b> The results of this study indicated that the antioxidant activity value of the IC<sub>50</sub> ethanol extract was 12.720±0.12 μg mL<sup>1</sup> and ascorbic acid (vitamin C) as a standard control was 3.14±0.12 μg mL<sup>1</sup>. The TNF- α normal group (7.83 pg mL<sup>1</sup>), negative control (250.92 pg mL<sup>1</sup>), positive control (123.66 pg mL<sup>1</sup>), treatment group dose 200 (192.20 pg mL<sup>1</sup>), 300 (97, 95 pg mL<sup>1</sup>) and 400 mg kg<sup>1</sup> b.wt. (28.78 pg mL<sup>1</sup>). <b>Conclusion:</b> This study concluded that the ethanol extract of the stem of <i>E. rubroloba</i> has a very strong antioxidant activity and is an anti-inflammatory which is the best in reducing levels of TNF-α at a dose of 400 mg kg<sup>1</sup> b.wt.

Keywords: E. rubroloba; FRAP method; Tumor Necrosis Factor-Alpha (TNF-α ); anti-inflammatory; antioxidants; ethanol extract; in vivo.