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. 2022 Jun 7;21(2):1317-1326.
doi: 10.1007/s40200-022-01061-9. eCollection 2022 Dec.

The roles of MTHFR (C677T, A1298C) and MGP (G-7A, T-138C) gene variations in development of diabetic nephropathy in patients with type 2 diabetes mellitus

Arzu Ay  1 , Nevra Alkanli  2 , Idris Kurt  3 , Sedat Ustundag  4 , Tammam Sipahi  1 , Necdet Sut  5
Affiliations

The roles of MTHFR (C677T, A1298C) and MGP (G-7A, T-138C) gene variations in development of diabetic nephropathy in patients with type 2 diabetes mellitus

Arzu Ay et al. J Diabetes Metab Disord. .

Abstract

Background: The goal of this study is to determine the role of MTHFR (C677T, A1298C) and MGP (G-7A, T-138C) gene variations in DN development.

Methods: There were 61 DN patients and 55 healthy controls in this study. The genotype distributions of these gene variations were determined using PCR and RFLP methods.

Results: According to our study, there was no significant relationship between these gene variations and DN (p > 0.05). The allele frequencies of the MTHFR C677T gene variation in the control group were found significantly different from the Hardy-Weinberg distribution (p < 0.05). According to combined genotype analysis, GA-TT combined genotype of MGP (G-7A/T-138C) gene variations was observed significantly more in the patient group with DN. The GA-TT combined genotype of MGP (G-7A/T-138C) gene variations was differ significantly between these groups (OR: 2.359, %95 CI: 1.094-5.087, p = 0.028).

Conclusion: In our study, although MTHFR and MGP gene variations were not risk factors, the GA-TT combined genotype of the MGP (G-7A/T-138C) gene variation was detected as an important risk factor for DN.

Keywords: A1298C) gene variations; Diabetic nephropathy; MGP (G-7A; MTHFR (C677T; PCR; RFLP; T-138C) gene variations.

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Conflict of interest statement

Conflict of interestThe authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
MTHFR C6777 gene variation genotype distributions
Fig. 2
Fig. 2
MTHFR A1298C gene variation genotype distributions
Fig. 3
Fig. 3
MGP G-7A gene variation genotype distributions
Fig. 4
Fig. 4
MGP T-138C gene variation genotype distributions
See this image and copyright information in PMC

References

    1. Guasch-Ferré M, Hruby A, Toledo E, Clish CB, Martínez-González MA, Salas-Salvadó J, Hu FB. Metabolomics in prediabetes and diabetes: a systematic review and Meta-analysis. Diabetes Care. 2016;39(5):833–846. doi: 10.2337/dc15-2251. - DOI - PMC - PubMed
    1. Arneth B, Arneth R, Shams M. Metabolomics of type 1 and type 2 diabetes. Int J Mol Sci. 2019;20(10):2467. doi: 10.3390/ijms20102467. - DOI - PMC - PubMed
    1. Xue R, Gui D, Zheng L, Zhai R, Wang F, Wang N. Mechanistic insight and Management of Diabetic Nephropathy: recent Progress and future perspective. J Diabetes Res. 2017;2017:1839809. doi: 10.1155/2017/1839809. - DOI - PMC - PubMed
    1. Thomas MC, Cooper ME, Zimmet P. Changing epidemiology of type 2 diabetes mellitus and associated chronic kidney disease. Nat Rev Nephrol. 2016;12(2):73–81. doi: 10.1038/nrneph.2015.173. - DOI - PubMed
    1. Gupta A, Sharma S, Lakkakula S, Bhaskar LV. Association between the methylenetetrahydrofolate reductase (MTHFR) gene 677C>T and 1298A>C polymorphisms and the risk of diabetic nephropathy; a meta-analysis. J Renal Inj Prev. 2019;8(3):175–184. doi: 10.15171/jrip.2019.33. - DOI

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