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. 2022 Mar 18;21(2):1261-1270.
doi: 10.1007/s40200-022-01012-4. eCollection 2022 Dec.

Cardioprotective effect of Hrudroga Chintamani Rasa in isoproterenol induced cardiotoxicity in male Sprague Dawley rats

Ankit P Laddha  1 , Mukesh B Chawda  2 , Yogesh A Kulkarni  1
Affiliations

Cardioprotective effect of Hrudroga Chintamani Rasa in isoproterenol induced cardiotoxicity in male Sprague Dawley rats

Ankit P Laddha et al. J Diabetes Metab Disord. .

Erratum in

Abstract

Purpose: Ayurvedic system, a traditional medicinal system has mentioned a preparation Bruhat Vata Chintamani Rasa (Suvarnayukta) for management of heart diseases. Hrudroga Chintamani Rasa (HCR) is a formulation containing Bruhat Vata Chintamani Rasa and a few additional ingredients having beneficial effects in heart diseases. The present study was designed to investigate the cardioprotective activity of the Hrudroga Chintamani Rasa in isoproterenol (ISO)-induced myocardial infarction in rats.

Methods: Male Sprague Dawley rats were treated with HCR at a dose of 56.16 and 112.32 mg/kg for 30 days. Animals received ISO (85 mg/kg. s.c.) on 28th and 29th day at an interval of 24 h.

Result: Disease control animals treated with HCR at a dose of 56.16 mg/kg and 112.32 mg/kg to rats showed a significant reduction in elevated levels of aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and creatine phosphokinase MB (CK-MB), and prevented loss of depleted antioxidant enzymes from the cardiac tissue. ISO-altered electrocardiogram pattern and haemodynamic parameters were also brought about to normal by treatment with HCR. HCR treatment also improved the levels of 5' adenosine monophosphate-activated protein kinase (AMPK) and Silent information regulator 1 (SIRT1) which have potent role in antioxidant defence mechanism. Histopathological findings also showed HCR treatment prevented cardiac tissue from damage.

Conclusion: HCR treatment showed a significant cardioprotective effect in ISO-induced cardiotoxicity in rats probably because of the potent antioxidant activity.

Supplementary information: The online version contains supplementary material available at 10.1007/s40200-022-01012-4.

Keywords: Ayurvedic system; Cardio toxicity; Cardiovascular; Hrudroga Chintamani Rasa; Isoproterenol; Myocardial infarction.

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Conflict of interest statement

Conflict of interestThe author(s) declared the following potential conflict of interest with respect to the research, authorship, and/or publication of this article: One of the co-author is from Shree Dhootapapeshwar Limited, which has funded the study. The first and corresponding author declare no conflict of interest.

Figures

Fig. 1
Fig. 1
Effect of HCR on systolic blood pressure, diastolic pressure and mean arterial blood pressure. All values are expressed as Mean ± S.E.M. (n = 6). **p < 0.01 and *p < 0.05 when compared with disease control. ###p < 0.001 compared with normal control
Fig. 2
Fig. 2
Effect of HCR on left ventricular end-diastolic pressure (LVEDP) and rate of ventricular contractility (+ dp/dt and –dp/dt). All values are expressed as Mean ± S.E.M. (n = 6). ***p < 0.001, **p < 0.01 and *p < 0.05 when compared with disease control. ###p < 0.001 compared with normal control
Fig. 3
Fig. 3
Effect of HCR on biochemical parameters. All values are expressed as Mean ± S.E.M. (n = 6). ***p < 0.001, **p < 0.01 and *p < 0.05 when compared with disease control. ###p < 0.001 compared with normal control
Fig. 4
Fig. 4
Effect of HCR on 5' adenosine monophosphate-activated protein kinase (AMPK) and Silent information regulator 1 (SIRT1) levels. All values are expressed as Mean ± S.E.M. (n = 6). *p < 0.05 when compared with disease control. ###p < 0.001 and ##p < 0.01 compared with normal control
Fig. 5
Fig. 5
Effect of HCR on cardiac troponin (cTn-I) level. All values are expressed as Mean ± S.E.M. (n = 6). *p < 0.05 when compared with disease control. ###p < 0.001 and ##p < 0.01 compared with normal control
Fig. 6
Fig. 6
Effect of HCR on cardiac histopathology (H& E Staining, 100X) N Tissue necrosis, D Degeneration, A Angiogenesis, L Lymphocytic infiltration, C Collagen cardiac muscle
Fig. 7
Fig. 7
Effect of HCR on cardiac histopathology (Masson-Trichrome Staining, 400X)
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