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Meta-Analysis
. 2022 Nov 4:13:972247.
doi: 10.3389/fimmu.2022.972247. eCollection 2022.

Mesenchymal stromal cell extracellular vesicles for multiple sclerosis in preclinical rodent models: A meta-analysis

Chengfeng Xun  1   2   3 , Huiyin Deng  4 , Jing Zhao  1 , Lite Ge  1   2   3 , Zhiping Hu  1
Affiliations
Meta-Analysis

Mesenchymal stromal cell extracellular vesicles for multiple sclerosis in preclinical rodent models: A meta-analysis

Chengfeng Xun et al. Front Immunol. .

Abstract

Introduction: Extracellular vesicles (EVs), especially mesenchymal stem (stromal) cell-derived EVs (MSC-EVs), have gained attention as potential novel treatments for multiple sclerosis (MS). However, their effects remain incompletely understood. Thus, the purpose of this meta-analysis was to systematically review the efficacy of MSC-EVs in preclinical rodent models of MS.

Methods: We searched PubMed, EMBASE, and the Web of Science databases up to August 2021 for studies that reported the treatment effects of MSC-EVs in rodent MS models. The clinical score was extracted as an outcome. Articles were peer-reviewed by two authors based on the inclusion and exclusion criteria. This meta-analysis was conducted using Stata 15.1 and R.

Results: A total of twelve animal studies met the inclusion criteria. In our study, the MSC-EVs had a positive overall effect on the clinical score with a standardized mean difference (SMD) of -2.17 (95% confidence interval (CI)):-3.99 to -0.34, P = 0.01). A significant amount of heterogeneity was observed among the studies.

Conclusions: This meta-analysis suggests that transplantation of MSC-EVs in MS rodent models improved functional recovery. Additionally, we identified several critical knowledge gaps, such as insufficient standardized dosage units and uncertainty regarding the optimal dose of MSC-EVs transplantation in MS. These gaps must be addressed before clinical trials can begin with MSC-EVs.

Keywords: animal model; exosomes; extracellular vesicles; meta-analysis; multiple sclerosis; stem cells; systematic review.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
PRISM Aflow diagram for review and selection process of studies included in meta-analysis of MSC-EVs in rodent models of MS.
Figure 2
Figure 2
Forest plot shows the mean effect size and 95% confidence interval (CI) for clinical score.
Figure 3
Figure 3
Sensitivity analysis of the studies included in clinical score.
Figure 4
Figure 4
The evaluation of publication bias. (A) Funnel plots for clinical score, with the y-axis signifying study quality and the x-axis showing the study results. (B) Trim-and-fill method was used to evaluate the missing studies in clinical score. SMD, standardized mean difference.
Figure 5
Figure 5
Clinical challenges of MSC-EVs therapy for MS.
See this image and copyright information in PMC

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References

    1. Filippi M, Bar-Or A, Piehl F, Preziosa P, Solari A, Vukusic S, et al. . Multiple sclerosis. Nat Rev Dis Primers (2018) 4(1):43. doi: 10.1038/s41572-018-0041-4 - DOI - PubMed
    1. Jacques FH. Defining the clinical course of multiple sclerosis: the 2013 revisions. Neurology (2015) 84(9):963. doi: 10.1212/01.wnl.0000462309.76486.c5 - DOI - PubMed
    1. Bjelobaba I, Begovic-Kupresanin V, Pekovic S, Lavrnja I. Animal models of multiple sclerosis: Focus on experimental autoimmune encephalomyelitis. J Neurosci Res (2018) 96(6):1021–42. doi: 10.1002/jnr.24224 - DOI - PubMed
    1. American Association of Neurological Surgeons. American Society of Neuroradiology. Cardiovascular and Interventional Radiology Society of Europe. Canadian Interventional Radiology Association. Congress of Neurological Surgeons. European Society of Minimally Invasive Neurological Therapy et al. . Multisociety consensus quality improvement revised consensus statement for endovascular therapy of acute ischemic stroke. Int J Stroke (2018) 13(6):612–32. doi: 10.1177/1747493018778713 - DOI - PubMed
    1. Nicholas RS, Rhone EE, Mariottini A, Silber E, Malik O, Singh-Curry V, et al. . Autologous hematopoietic stem cell transplantation in active multiple sclerosis: A real-world case series. Neurology (2021) 97(9):e890–901. doi: 10.1212/WNL.0000000000012449 - DOI - PMC - PubMed

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