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. 2022 Nov 10:2022:7490691.
doi: 10.1155/2022/7490691. eCollection 2022.

Quantitative NMR Interpretation without Reference

Priscila Ivo Rubim de Santana  1   2 , Joyce Sobreiro Francisco Diz de Almeida  1 , Tanos Celmar Costa França  1   3 , Jochen Junker  2
Affiliations

Quantitative NMR Interpretation without Reference

Priscila Ivo Rubim de Santana et al. J Anal Methods Chem. .

Abstract

As has been documented numerous times over the years, nuclear magnetic resonance (NMR) experiments are intrinsically quantitative. Still, quantitative NMR methods have not been widely adopted or largely introduced into pharmacopoeias. Here, we describe the quantitative interpretation of the 1D proton NMR experiment using only absolute signal intensities with the variation of common experimental parameters and their application.

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Conflict of interest statement

The authors declare that there are no conflicts of interest.

Figures

Figure 1
Figure 1
Quinine (1, C20 H24 N2 O2, MW 324.42 g/mol), used for the qNMR application.
Figure 2
Figure 2
1H NMR spectrum of Quinine (1), the used integration limits are delimited. Further signals were not used due to overlap and complex coupling patterns.
Figure 3
Figure 3
The average signal intensity increment was observed in dependence on sample concentration, the number of protons, and scans, separated for different RG values.
Figure 4
Figure 4
Back-calculated sample concentrations using not linearized RG values (original RG) and manually linearized RG values (linearized RG) plotted against the prepared concentrations.
See this image and copyright information in PMC

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