Genome Sequences of Two Lytic Staphylococcus aureus Bacteriophages Isolated from Wastewater

Joshua J Iszatt^{1

2}, Alexander N Larcombe^{1

2}, Luke W Garratt^{1

2}, Stephen M Stick^{2

3

4}, Patricia Agudelo-Romero^#^{2

5

6}, Anthony Kicic^#^{1

2

3

4}; WAERP; AREST CF

Affiliations

¹ Occupation, Environment and Safety, School of Population Health, Curtin University, Perth, Western Australia, Australia.
² Wal-yan Respiratory Research Centre, Telethon Kids Institute, The University of Western Australia, Perth, Western Australia, Australia.
³ Department of Respiratory and Sleep Medicine, Perth Children's Hospital, Nedlands, Western Australia, Australia.
⁴ Centre for Cell Therapy and Regenerative Medicine, School of Medicine and Pharmacology, The University of Western Australia and Harry Perkins Institute of Medical Research, Perth, Western Australia, Australia.
⁵ Australian Research Council Centre of Excellence in Plant Energy Biology, School of Molecular Sciences, The University of Western Australia, Perth, Western Australia, Australia.
⁶ European Virus Bioinformatics Center, Jena, TH, Germany.

^# Contributed equally.

PMID: 36409081
PMCID: PMC9753628
DOI: 10.1128/mra.00954-22

Genome Sequences of Two Lytic Staphylococcus aureus Bacteriophages Isolated from Wastewater

Joshua J Iszatt et al. Microbiol Resour Announc. 2022.

. 2022 Dec 15;11(12):e0095422.

doi: 10.1128/mra.00954-22. Epub 2022 Nov 21.

Authors

Joshua J Iszatt^{1

2}, Alexander N Larcombe^{1

2}, Luke W Garratt^{1

2}, Stephen M Stick^{2

3

4}, Patricia Agudelo-Romero^#^{2

5

6}, Anthony Kicic^#^{1

2

3

4}; WAERP; AREST CF

Affiliations

¹ Occupation, Environment and Safety, School of Population Health, Curtin University, Perth, Western Australia, Australia.
² Wal-yan Respiratory Research Centre, Telethon Kids Institute, The University of Western Australia, Perth, Western Australia, Australia.
³ Department of Respiratory and Sleep Medicine, Perth Children's Hospital, Nedlands, Western Australia, Australia.
⁴ Centre for Cell Therapy and Regenerative Medicine, School of Medicine and Pharmacology, The University of Western Australia and Harry Perkins Institute of Medical Research, Perth, Western Australia, Australia.
⁵ Australian Research Council Centre of Excellence in Plant Energy Biology, School of Molecular Sciences, The University of Western Australia, Perth, Western Australia, Australia.
⁶ European Virus Bioinformatics Center, Jena, TH, Germany.

^# Contributed equally.

PMID: 36409081
PMCID: PMC9753628
DOI: 10.1128/mra.00954-22

Abstract

Two lytic double-stranded DNA (dsDNA) bacteriophages, belonging to the family Herelleviridae, were isolated from wastewater in Western Australia. Biyabeda-mokiny 2 appears to belong to the genus Kayvirus, and Koomba-kaat 1 to Silviavirus.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

References

1. Rasigade JP, Vandenesch F. 2014. Staphylococcus aureus: a pathogen with still unresolved issues. Infect Genet Evol 21:510–514. doi:10.1016/j.meegid.2013.08.018. - DOI - PubMed
1. Mulani MS, Kamble EE, Kumkar SN, Tawre MS, Pardesi KR. 2019. Emerging strategies to combat ESKAPE pathogens in the era of antimicrobial resistance: a review. Front Microbiol 10:539. doi:10.3389/fmicb.2019.00539. - DOI - PMC - PubMed
1. Lehman SM, Mearns G, Rankin D, Cole RA, Smrekar F, Branston SD, Morales S. 2019. Design and preclinical development of a phage product for the treatment of antibiotic-resistant Staphylococcus aureus infections. Viruses 11:88. doi:10.3390/v11010088. - DOI - PMC - PubMed
1. Jakočiūnė D, Moodley A. 2018. A rapid bacteriophage DNA extraction method. Methods Protoc 1:27. doi:10.3390/mps1030027. - DOI - PMC - PubMed
1. Bolger AM, Lohse M, Usadel B. 2014. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 30:2114–2120. doi:10.1093/bioinformatics/btu170. - DOI - PMC - PubMed

Grants and funding

LinkOut - more resources

Full Text Sources

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Genome Sequences of Two Lytic Staphylococcus aureus Bacteriophages Isolated from Wastewater

Affiliations

Genome Sequences of Two Lytic Staphylococcus aureus Bacteriophages Isolated from Wastewater

Authors

Affiliations

Abstract

Conflict of interest statement

References

Grants and funding

LinkOut - more resources

Full Text Sources