Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Apr;111(4):872-880.
doi: 10.1002/jbm.b.35197. Epub 2022 Nov 24.

Antimicrobial activity and cytotoxicity study of cerium oxide nanoparticles with two different sizes

Svetlana Yefimova  1 , Vladimir Klochkov  1 , Nataliya Kavok  1 , Anton Tkachenko  2 , Anatolii Onishchenko  2 , Tatyana Chumachenko  2   3 , Nadir Dizge  4 , Sadin Özdemir  5 , Serpil Gonca  6 , Kasim Ocakoglu  7
Affiliations

Antimicrobial activity and cytotoxicity study of cerium oxide nanoparticles with two different sizes

Svetlana Yefimova et al. J Biomed Mater Res B Appl Biomater. 2023 Apr.

Abstract

The control over bacterial diseases requires the development of novel antibacterial agents. The use of antibacterial nanomedicines is one of the strategies to tackle antibiotic resistance. The study was designed to assess the antimicrobial activity of cerium oxide (CeO2 ) nanoparticles (NP) of two different sizes (CeO2 NP1 [1-2 nm] and CeO2 NP2 [10-12 nm]) and their cytotoxicity towards eukaryotic cells. The antimicrobial activity, effects of nanoparticles on DNA cleavage, microbial cell viability, and biofilm formation inhibition were analyzed. The impact of cerium oxide nanoparticles on eryptosis of erythrocytes was estimated using annexin V staining by flow cytometry. The newly synthesized CeO2 NP1 and CeO2 NP2 displayed moderate antimicrobial activities. CeO2 NP1 and CeO2 NP2 exhibited single-strand DNA cleavage ability. CeO2 NPs were found to show 100% microbial cell viability inhibition at a concentration of 500 mg/L. In addition, CeO2 NP1 and CeO2 NP2 inhibited the biofilm formation of S. aureus and P. aeruginosa. Larger cerium oxide nanoparticles were found to be less toxic against erythrocytes compared with the smaller ones. CeO2 nanoparticles demonstrate moderate antimicrobial activity and low cytotoxicity towards erythrocytes, which make them promising antibacterial agents.

Keywords: DNA cleavage; antimicrobial; biofilm inhibition; cerium; microbial cell viability; nanoparticle.

PubMed Disclaimer

References

REFERENCES

    1. Manyi-Loh C, Mamphweli S, Meyer E, Okoh A. Antibiotic use in agriculture and its consequential resistance in environmental sources: potential public health implications. Molecules. 2018;23(4):795. doi:10.3390/molecules23040795
    1. Landers TF, Cohen B, Wittum TE, Larson EL. A review of antibiotic use in food animals: perspective, policy, and potential. Public Health Rep. 2012;127(1):4-22. doi:10.1177/003335491212700103
    1. Ventola CL. The antibiotic resistance crisis: part 1: causes and threats. Pharm Therapeut. 2015;40(4):277-283.
    1. Piewngam P, Chiou J, Chatterjee P, Otto M. Alternative approaches to treat bacterial infections: targeting quorum-sensing. Expert Rev Anti Infect Ther. 2020;18(6):499-510. doi:10.1080/14787210.2020.1750951
    1. Lei J, Sun L, Huang S, et al. The antimicrobial peptides and their potential clinical applications. Am J Transl Res. 2019;11(7):3919-3931.

Publication types

MeSH terms

LinkOut - more resources